乌蛇驱风汤及诸加减方对豚鼠慢性湿疹模型的疗效比较

发布时间：2019-03-11 09:45

【摘要】：目的:比较乌蛇驱风汤及诸加减方对豚鼠慢性湿疹的疗效。方法:健康豚鼠58只,随机抽取8只,雌雄各半,作为正常组,将其余的50只豚鼠参照进行造模,背部剃毛,小剂量2,4-二硝基氯苯为抗原,多次刺激豚鼠皮肤制备慢性湿疹模型,将造模成功的48只豚鼠分雌雄随机分为6组,分别为1号方组(乌蛇驱风汤原方,8.05 g·kg-1),2号方组(7.22 g·kg-1),3号方组11.75 g·kg-1,4号方组(12.86 g·kg-1),丁酸氢化可的松乳膏组(0.5 g·kg-1)、模型组,口服ig给药,连续给药7 d,末次用药后24 h,处死所有实验动物,根据皮损局部的症状评分和皮损处的病理改变进行疗效评估。结果:与正常组比较,模型组病理症状评分明显升高(P0.05),模型组皮损处的病理表现仍呈现表皮和真皮水肿,表皮层增厚,以棘层细胞增生为主,真皮浅层血管轻度扩张,周围有以淋巴细胞为主的炎症细胞浸润;与模型组比较,1号方组,2号方组,3号方组,4号方组及丁酸氢化可的松乳膏组病理症状评分明显降低(P0.05),乌蛇驱风汤组及丁酸氢化可的松乳膏组皮损组织病理改变较模型组明显改善,与氢化可的松组病理表现相似;各乌蛇驱风汤加减方组组织病理表现较模型组改善不明显。结论:乌蛇驱风汤对豚鼠慢性湿疹的疗效优于诸加减方,与丁酸氢化可的松乳膏组疗效相当。
[Abstract]:Objective: to compare the therapeutic effect of Wuhe Qifeng decoction and Zhujijianfang on chronic eczema in guinea pigs. Methods: 58 healthy guinea pigs were randomly selected as normal control group. The remaining 50 guinea pigs were treated with back shaving with a low dose of 2, 4-dinitrochlorobenzene as antigen. 48 guinea pigs with chronic eczema were randomly divided into 6 groups: group 1 (the original recipe of Wuhe Qufeng decoction, 8.05 g kg-1), and the other 48 guinea pigs were randomly divided into 6 groups: group 1 (the original recipe of Wuhe Qufeng decoction, 8.05 g / min). Group 2 (7.22 g kg-1), group 3 (11.75 g kg-1,4) (12.86 g kg-1), hydrocortisone butyrate cream group (0.5 g kg-1), model group, oral administration of ig. After 7 days of continuous administration and 24 hours after the last administration, all the animals were killed and evaluated according to the local symptom score and pathological changes of the lesions. Results: compared with the normal group, the scores of pathological symptoms in the model group were significantly higher (P0.05). The pathological manifestations of the skin lesions in the model group still showed epidermis and dermis edema, the thickness of the epidermis layer was thickened, and the proliferation of spinous layer cells was the main factor. The superficial dermal vessels were slightly dilated with lymphocyte-dominated inflammatory cells infiltrating around them. Compared with the model group, the scores of pathological symptoms in group 1, group 2, group 3, group 4 and hydrocortisone butyrate were significantly lower than those in group 1, group 2, group 3, group 4 and hydrocortisone butyrate cream group (P0.05). Compared with the model group, the pathological changes of the skin lesions in the two groups were significantly improved, which were similar to those in the hydrocortisone group. The pathological changes of the two groups were similar to those of the hydrocortisone group. Compared with the model group, the histopathological manifestation of Guowu snake-dispelling decoction group was not significantly improved. Conclusion: the curative effect of Wuhe Qifeng decoction on chronic eczema of guinea pig is better than that of Zhijianfang, which is similar to that of hydrocortisone butyrate cream group.
【作者单位】：安徽中医药高等专科学校;芜湖市中医院;
【基金】：省级优秀青年人才基金项目(2009SQRZ215)
【分类号】：R285.5;R-332

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