人源重要功能新基因的克
发布时间：2021-08-07 09:21
22周龄人胎肝是胎儿造血的主要场所,此阶段是造血系统迁入和迁出的关键转折点。这一独特性使其极具研究价值。基于对22周龄人胎肝cDNA进行大规模测序的结果,采用生物信息学方法和实验手段（cDNA末端快速扩增法等）获得了13条具有重要功能线索的人源新基因的22条全长cDNA序列。所有这些序列均率先在GenBank注册。关于其中部分基因的主要研究结果如下: 人源Semaphorin 6C和Semaphorin 6D:克隆了Semaphorin基因家族的两个新成员SEMA6C和SEMA6D,它们都属于膜结合型的第六亚家族。这两个基因分别由20个和19个外显子组成,染色体定位分别为1q12-21.1和15q21.1。关于SEMA6C和SEMA6D,分别发现了3种和5种不同剪切形式,它们的表达与组织特异性及发育阶段有关。Northern杂交结果显示,SEMA6C主要在骨骼肌中表达,SEMA6D则在肾、脑和胎盘中高表达,在心肌和骨骼肌中也有一定量的表达。缺失体研究表明,其胞外结构域（Sema结构域和PSI结构域）的完整性是Semaphorin蛋白进行适当的翻译后修饰和亚细胞定位所必须的。SEM...
【文章来源】：中国人民解放军军事科学院北京市
【文章页数】：120 页
【学位级别】：博士
【文章目录】：
中文摘要
英文摘要
Chapter 1 Identification, Characterization and Functional Study of the Two Novel Human Members of Semaphorin Gene Family
1. Introduction
2. Materials and Methods
3. Results
3.1 Identification and Cloning of Human Semaphorin SEMA6C and SEMA6D
3.2 The Genomic Structures and Chromosomal Localization of the Genes for Human SEMA6C and SEMA6D
3.3 Distribution of SEMA6C and SEMA6D mRNAs
3.4 Alternative Splicing of SEMA6C and SEMA6D
3.5 Characterization of SEMA6C and SEMA6D
3.6 Growth Cone Collapse Activity of SEMA6C and SEMA6D
4. Discussion
References
Chapter 2: Characterization and Expression of Three Novel Differentiation-related Genes Belong to the Human NDRG Gene Family
1. Introduction
2. Materials and Methods
3. Results
3.1 Identification and cloning of human genes NDRG2, NDRG3 and NDRG4
3.2 Sequence analysis of human NDRG2, NDRG3 and NDRG4 proteins
3.3 Chromosomal localization of the genes for human NDRG2, NDRG3 and NDRG4
3.4 Transcription profile of NDRG2, NDRG3 and NDRG4
3.5 Subcellular localization of NDRG2, NDRG3 and NDRG4 proteins
4. Discussion
References
Chapter 3: Characterization and Tissue Expression of a Novel Human Gene npdc1
1. Introduction
2. Materials and Methods
3. Results and Discussion
3.1 Identification and cloning of hnpdcl
3.2 Sequence analysis of hNPDCl
3.3 Chromosomal localization of the gene for hnpdcl
3.4 Transcription profile of the hnpdcl gene in different human tissues
References
Chapter 4: Cloning, Chromosomal Assignment and Tissue Expression of a Novel Human Protein Tyrosine Phosphatase Gene
1. Introduction
2. Materials and Methods
3. Results and Discussion
3.1 Identification and cloning of human PTP-TD14 gene
3.2 Sequence analysis of human PTP-TD14
3.3 Transcription profile of the human PTP-TD14 gene
3.4 Chromosomal localization of the genes for human PTP-TD14
References
Chapter 5: Cloning and Chromosomal Assignment of Six Novel Human Genes with Important Biological Functions
1. Introduction
2. Materials and Methods
3. Results and Discussion
3.1 Human BKLF gene
3.2 Human c-Jun binding protein (JBP) gene
3.3 Human hematopoietic zinc finger protein (Hzf) gene
3.4 Human liver membrane-bound protein (LMP) gene
3.5 Human liver nuclear protein LNP gene
3.6 Human clathrin coat assembly protein (AP47) gene
References
综述 Semaphorin及其受体的研究进展
附录一 英文缩写名
附录二 22 full-length cDNAs deposited in GenBank
附录三 博士后期间申请(负责)的基金项目和专利项目
附录四 博士后期间完成的论文
致谢
本文编号：3327530
【文章来源】：中国人民解放军军事科学院北京市
【文章页数】：120 页
【学位级别】：博士
【文章目录】：
中文摘要
英文摘要
Chapter 1 Identification, Characterization and Functional Study of the Two Novel Human Members of Semaphorin Gene Family
1. Introduction
2. Materials and Methods
3. Results
3.1 Identification and Cloning of Human Semaphorin SEMA6C and SEMA6D
3.2 The Genomic Structures and Chromosomal Localization of the Genes for Human SEMA6C and SEMA6D
3.3 Distribution of SEMA6C and SEMA6D mRNAs
3.4 Alternative Splicing of SEMA6C and SEMA6D
3.5 Characterization of SEMA6C and SEMA6D
3.6 Growth Cone Collapse Activity of SEMA6C and SEMA6D
4. Discussion
References
Chapter 2: Characterization and Expression of Three Novel Differentiation-related Genes Belong to the Human NDRG Gene Family
1. Introduction
2. Materials and Methods
3. Results
3.1 Identification and cloning of human genes NDRG2, NDRG3 and NDRG4
3.2 Sequence analysis of human NDRG2, NDRG3 and NDRG4 proteins
3.3 Chromosomal localization of the genes for human NDRG2, NDRG3 and NDRG4
3.4 Transcription profile of NDRG2, NDRG3 and NDRG4
3.5 Subcellular localization of NDRG2, NDRG3 and NDRG4 proteins
4. Discussion
References
Chapter 3: Characterization and Tissue Expression of a Novel Human Gene npdc1
1. Introduction
2. Materials and Methods
3. Results and Discussion
3.1 Identification and cloning of hnpdcl
3.2 Sequence analysis of hNPDCl
3.3 Chromosomal localization of the gene for hnpdcl
3.4 Transcription profile of the hnpdcl gene in different human tissues
References
Chapter 4: Cloning, Chromosomal Assignment and Tissue Expression of a Novel Human Protein Tyrosine Phosphatase Gene
1. Introduction
2. Materials and Methods
3. Results and Discussion
3.1 Identification and cloning of human PTP-TD14 gene
3.2 Sequence analysis of human PTP-TD14
3.3 Transcription profile of the human PTP-TD14 gene
3.4 Chromosomal localization of the genes for human PTP-TD14
References
Chapter 5: Cloning and Chromosomal Assignment of Six Novel Human Genes with Important Biological Functions
1. Introduction
2. Materials and Methods
3. Results and Discussion
3.1 Human BKLF gene
3.2 Human c-Jun binding protein (JBP) gene
3.3 Human hematopoietic zinc finger protein (Hzf) gene
3.4 Human liver membrane-bound protein (LMP) gene
3.5 Human liver nuclear protein LNP gene
3.6 Human clathrin coat assembly protein (AP47) gene
References
综述 Semaphorin及其受体的研究进展
附录一 英文缩写名
附录二 22 full-length cDNAs deposited in GenBank
附录三 博士后期间申请(负责)的基金项目和专利项目
附录四 博士后期间完成的论文
致谢
本文编号：3327530
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