依达拉奉对大鼠小肠缺血-再灌注所致肺损伤的保护作用

发布时间：2018-01-20 13:55

本文关键词： 小肠缺血-再灌注损伤肺依达拉奉　出处：《临床麻醉学杂志》2017年04期 　论文类型：期刊论文

【摘要】：目的探讨依达拉奉对大鼠小肠缺血-再灌注所致肺损伤的保护作用。方法雄性SD大鼠18只,随机均分为假手术组(Sham组),缺血-再灌注组(IR组)和依达拉奉组(E组)。Sham组只分离肠系膜上动脉,不做其他处理;IR组分离肠系膜上动脉,从大鼠尾静脉注射与E组等量的生理盐水后,用无创动脉夹夹闭120min后移去动脉夹,再灌注120min;E组在缺血-再灌注前静脉注射依达拉奉6mg/kg。再灌注120min后采集标本。肺组织HE染色后病理学检测,采集腹主动脉血液检测大鼠血清中TNF-α和IL-6浓度,取肺组织检测髓过氧化物酶(MPO)活性和丙二醛(MDA)浓度。结果与Sham组比较,IR组肺泡上皮细胞广泛水肿、炎性细胞浸润、肺泡肺萎陷、肺毛细血管扩张出血;E组肺组织病理改变较IR组明显改善,肺泡炎性渗出减少;E组病理评分为(2.1±0.7)分,明显低于IR组的(5.7±1.1)分,IR组病理评分明显高于Sham组的(1.5±0.2)分(P0.01);血清中TNF-α和IL-6的浓度明显少于IR组,肺组织中MPO活性和MDA浓度明显低于IR组(P0.01)。结论依达拉奉能够明显改善小肠缺血-再灌注性肺损伤。
[Abstract]:Objective to investigate the protective effect of Edaravone on small intestinal ischemia-reperfusion injury in rats. Methods 18 male SD rats were randomly divided into sham-operated group (Sham group). Ischemia-reperfusion group (IR group) and Edaravone group (E group) and group E (Sham group only separated superior mesenteric artery without other treatment; The superior mesenteric artery was isolated in IR group. The superior mesenteric artery was injected into the tail vein of rats with the same amount of normal saline as that in group E. The artery clamp was removed by non-invasive artery clamping for 120 minutes and then was perfused for 120 minutes. In group E, 6 mg / kg Edaravone was injected intravenously before ischemia-reperfusion. After 120 minutes of reperfusion, the specimens were collected, and the pathological changes of lung tissue were detected after HE staining. The concentrations of TNF- 伪 and IL-6 in serum of rats were measured by collecting the blood of abdominal aorta. The activity of myeloperoxidase (MPO) and the concentration of malondialdehyde (MDA) in lung tissue were measured. The results were compared with those in Sham group. In IR group, alveolar epithelial cells were widely edema, inflammatory cells infiltrated, alveolar lung collapse, pulmonary capillary dilatation and hemorrhage. The pathological changes of lung tissue in group E were significantly improved than those in group IR, and the alveolar inflammatory exudation was decreased. The pathological score of group E was 2.1 卤0.7, which was significantly lower than that of IR group (5.7 卤1.1). The pathological score of IR group was significantly higher than that of Sham group (1.5 卤0.2) (P 0.01). The concentrations of TNF- 伪 and IL-6 in serum were significantly lower than those in IR group. The activity of MPO and the concentration of MDA in lung tissue were significantly lower than those in IR group (P 0.01). Conclusion Edaravone can significantly improve intestinal ischemia-reperfusion lung injury.
【作者单位】：上海市浦东新区周浦医院医院麻醉科;
【基金】：上海市浦东新区科技发展基金(PKJ2015-Y40) 上海市浦东新区卫生系统学科带头人培养计划(PWRd2014-19)
【分类号】：R614
【正文快照】： 小肠缺血-再灌注(ischemia-reperfusion,I-R)损伤后呼吸衰竭是导致患者出现并发症和死亡的最常见原因[1],氧自由基、各种细胞因子(如TNF-α和IL-6)、内源性毒素、血小板激活因子及嗜中性粒细胞在小肠I-R损伤的病理生理中发挥了很重要的作用[2]。临床研究和动物实验表明,肠I-R

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