联合应用BMP-2及细胞分化抑制因子1基因延缓兔腰椎间盘退变的动物实验研究

发布时间：2018-01-22 00:33

  本文关键词： 椎间盘退变 基因治疗 BMP- 细胞分化抑制因子　出处：《中国修复重建外科杂志》2017年01期 　论文类型：期刊论文

【摘要】：目的研究兔腰椎间盘髓核内注射慢病毒携带的BMP-2和细胞分化抑制因子1(inhibitor of differentiation 1,Id1)基因能否有效改善椎间盘退变进程。方法取4月龄新西兰兔32只,体质量2.0~2.5 kg;以L3、4、L4、5、L5、6为目标椎间盘,经腹细针穿刺法制造椎间盘退变模型。将30只造模成功的实验动物随机分为5组,每组6只;造模后4周于各组目标椎间盘中分别注射Lv-BMP-2病毒(A组)、Lv-BMP-2+Lv-Id1病毒(B组)、LvId1病毒(C组)、Lv-绿色荧光蛋白空载体病毒(D组)及PBS(E组)。于注射后2、4、8周各组分别随机取2只动物行T2-mapping MRI检查获得确切的T2弛豫时间(T2值);之后处死动物取椎间盘组织,应用实时荧光定量PCR、ELISA法检测Ⅱ型胶原及蛋白聚糖的m RNA相对表达量及含量。结果 T2-mapping MRI检查示,注射后0、2周各组间T2值比较差异均无统计学意义(P0.05);注射后4周,A、B组T2值显著高于C、D、E组(P0.05),A、B组间比较差异无统计学意义(P0.05);注射后8周,B组T2值显著高于其余各组(P0.05)。实时荧光定量PCR及ELISA检测示,注射后2、4、8周B组Ⅱ型胶原及蛋白聚糖的m RNA相对表达量及含量均显著高于其余各组(P0.05)。结论联合应用BMP-2和Id1基因在体内实验中能有效延缓兔腰椎间盘退变的进程。
[Abstract]:Objective to study the intramedullary injection of lentivirus-carrying BMP-2 and cell differentiation inhibitor of differentiation 1 into the nucleus pulposus of lumbar intervertebral disc in rabbits. Methods 32 New Zealand rabbits (4 months old) with 2.0 kg body weight and 2.5 kg / kg were used to improve the degenerative process of intervertebral disc. The model of intervertebral disc degeneration was established with L3, L4, L4, L5, L5 and 6 as the target disc. The 30 successful experimental animals were randomly divided into 5 groups, 6 in each group, and 6 in each group were randomly divided into 5 groups, 6 rats in each group, and 6 animals in each group were randomly divided into 5 groups. After 4 weeks of modeling, Lv-BMP-2 virus group A was injected into the target disc of each group, respectively. Group B was injected with Lv-BMP-2 Lv-Id1 virus group B and group C was treated with LvId1 virus. Lv-green fluorescent protein empty vector virus group D) and PBS(E group. After 8 weeks, 2 animals were randomly selected for T2-mapping MRI to obtain the exact T 2 relaxation time and T 2 value. Then the animals were killed to take the intervertebral disc tissue and real-time fluorescence quantitative PCR was used. The relative expression and content of m RNA in type 鈪，

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