LARS人工韧带重建前交叉韧带:生物相容性及与骨组织的愈合

发布时间：2018-01-25 06:51

本文关键词：前交叉韧带假体和植入物组织工程组织构建 LARS人工韧带重建交叉韧带生物相容性骨组织苏木精-伊红染色 Masson染色扫描电镜诱导能力湖北省自然科学基金　出处：《中国组织工程研究》2017年16期 　论文类型：期刊论文

【摘要】：背景:LARS人工韧带能避免传统韧带引起的纤维间摩擦,为自体组织生长提供更多的空间。目的:探讨LARS人工韧带重建交叉韧带的生物相容性及与骨组织的愈合情况。方法:将20只山羊随机分为2组,保留残端组仅切断前交叉韧带股骨止点;切除残端组切除前交叉韧带的股骨、胫骨止点,以及上、下止点间的全部韧带组织与表面滑膜组织,两组均植入LARS人工韧带。植入后20个月,影像学观察韧带修复情况,扫描电镜观察纤维组织情况,组织学观察新生韧带生长情况。结果与结论:(1)影像学检查:CT检查显示,保留残端组股骨隧道扩大数显著高于切除残端组(P0.05),胫骨隧道扩大数显著低于切除残端组(P0.05);磁共振检查显示,LARS人工韧带位于股骨及胫骨隧道内和关节游离部分,在磁共振成像下T1、T2加权均为低信号,两组磁共振信号强度相同;(2)扫描电镜观察:两组均在LARS人工韧带之间存在明显的致密纤维组织,LARS人工韧带表面覆盖较多自体组织,高放大倍率下可见LARS人工韧带的自体纤维组织排列紧密、均匀;(3)组织学观察:苏木精-伊红染色显示,两组人工韧带在关节腔内游离部分和骨隧道内被大量自体组织包绕,呈长梭形排列,自体纤维组织整体与人工韧带包绕良好,呈无序排列,骨隧道部分与关节腔内游离部分存在成熟纤维组织,少量毛细血管浸润生长,未见坏死组织。Masson染色显示,两组LARS人工韧带骨隧道内部分和游离部分可见蓝色胶原包绕在人工韧带外部和内部,红染骨组织位于包裹胶原外部,未见明显过渡带;(4)结果表明:LARS人工韧带具备良好的生物相容性和纤维组织诱导能力,可促进骨组织愈合。
[Abstract]:Background\\\% LARS artificial ligament can avoid the friction between fibers caused by traditional ligaments. Objective: to study the biocompatibility of LARS artificial ligament for reconstruction of cruciate ligament and the healing of bone tissue. Methods: 20 goats were randomly divided into two groups. In the stump group, only the femoral stop point of anterior cruciate ligament was cut off. In the stump group, the femoral, tibial and surface synovial tissues between the anterior cruciate ligament (ACL) and the tibia were resected. The LARS artificial ligament was implanted in both groups 20 months after implantation. The repair of ligaments was observed by imaging, the fibrous tissue was observed by scanning electron microscope, and the growth of new ligaments was observed by histology. The number of femoral tunnel enlargement in the reserved stump group was significantly higher than that in the resected stump group (P 0.05), and the tibial tunnel expansion was significantly lower than that in the resected stump group (P 0.05). Magnetic resonance examination showed that the Lars artificial ligament was located in the femur and tibia tunnel and in the free part of the joint. The T _ 1T _ 2 weighted signal intensity of the two groups was the same as that of the control group. (2) scanning electron microscope observation: there was obvious dense fibrous tissue between the LARS artificial ligament and the surface of the LARS artificial ligament covered with more autologous tissue in both groups. At high magnification, the autofibril tissue of LARS artificial ligament was arranged closely and evenly. Histological observation: hematoxylin-eosin staining showed that the artificial ligaments of the two groups were wrapped by a large number of autologous tissues in the articular cavity and the bone tunnel and arranged in a long fusiform shape. The autologous fibrous tissue and artificial ligament were well wrapped and arranged disorderly. Mature fibrous tissue existed in the bone tunnel part and the free part of the articular cavity and a small amount of capillary infiltrated and grew. No necrotic tissue. Masson staining showed that the blue collagen was wrapped in the outer and inner parts of the artificial ligament in the two groups in the osseous tunnel and the free part of the LARS artificial ligament. The red-dyed bone tissue was located outside the collagen, and there was no obvious transitional zone. The results showed that the Lars artificial ligament had good biocompatibility and fibrous tissue induction ability, which could promote bone healing.
【作者单位】：三峡大学体育学院;长江大学医学院;
【基金】：湖北省自然科学基金面上项目(2015AFC331)~~
【分类号】：R318.1;R687.4
【正文快照】： 文章快速阅读:LARS人工韧带重建前交叉韧带的生物相容性及骨愈合保留残端组仅切断前交叉指标:结果:韧带股骨止点两组植入后20个月,LARS人工均植入影像学观察韧带韧带具备良LARS修复情况,扫描好的生物相切除残端组切除前交叉韧人工电镜观察纤维组容性和纤维带的股骨、胫骨止点,

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