糖皮质激素对人股骨头骨微血管内皮细胞功能的影响

发布时间：2018-01-29 08:10

本文关键词： 糖皮质激素内皮细胞基因芯片荧光定量PCR　出处：《中国矫形外科杂志》2015年15期 　论文类型：期刊论文

【摘要】：[目的]通过基因芯片对人股骨头骨微血管内皮细胞表达谱分析及荧光定量PCR验证,探讨糖皮质激素对骨微血管内皮细胞损伤后发生的功能变化。[方法]采用本实验室建立的方法进行人股骨头骨微血管内皮细胞的分离、培养及鉴定,证明细胞为骨微血管内皮细胞。用0.1 mg/ml的含氢化可的松的培养基培养细胞,建立糖皮质激素骨微血管内皮细胞损伤模型。设正常对照组,用晶芯湟mRNA表达谱芯片对细胞损伤模型和对照组进行差异性转录本检测,对差异性表达基因行q PCR验证。[结果]mRNA芯片表达基因筛选结果发现ICAM-1、ET-1受体、PAI-1、血管紧张素II受体较对照组明显上调,e NOS、ET-1、PGI2合成酶、VEGF、PGE合成酶及PGE受体表达明显下调。q PCR验证结果与芯片结果一致。[结论]糖皮质激素促进了人股骨头骨微血管内皮细胞分泌缩血管因子、促凝血的因子及相关受体的表达,降低了舒张血管因子及相应受体的表达。
[Abstract]:[Objective] to investigate the functional changes of human femoral head bone microvascular endothelial cells after injury induced by glucocorticoid (glucocorticoid) by means of gene chip analysis and fluorescence quantitative PCR verification. [Methods] Human femoral head bone microvascular endothelial cells were isolated, cultured and identified by the method established in our laboratory. It was proved that the cells were bone microvascular endothelial cells. The injury model of glucocorticoid bone microvascular endothelial cells was established with hydrocortisone containing hydrocortisone for 0. 1 mg/ml. The normal control group was set up. The differentially expressed genes were detected by using Jingxin Huang mRNA microarray and Q PCR in the model of cell injury and the control group. [Results: the results of mRNA microarray gene screening showed that ICAM-1 and angiotensin II receptors were significantly up-regulated compared with the control group. The expression of PGI2 synthase, VEGFG, PGE synthase and PGE receptor was significantly down-regulated. Q PCR confirmed that the results were consistent with those of microarray. [Conclusion: glucocorticoid can promote the expression of vasoconstriction factor, coagulation promoting factor and related receptors, and decrease the expression of vasodilator and its corresponding receptors in human femoral head bone microvascular endothelial cells.
【作者单位】：北京协和医学院研究生院;中日友好医院骨关节外科;中日友好医院临床医学研究所;
【基金】：国家自然科学基金资助项目(编号:81273972)
【分类号】：R96
【正文快照】： 非创伤性股骨头坏死是临床常见的难治性髋关节病变,Ikeuchi等[1]调查日本激素性股骨头坏死占1/3以上,在我国可能更多[2]。研究指出激素性股骨头坏死的病因是多方面原因综合作用的结果,包括骨细胞与成骨细胞凋亡,骨髓脂肪化骨内压增高,骨内微血管血栓形成以及内皮细胞的损伤。

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