过氧化物酶体增殖物PGC-1α对肝缺血再灌注损伤的保护作用研究

发布时间：2018-01-29 10:53

本文关键词： 过氧化物酶体增殖物受体γ共激活因子-α 肝再灌注损伤　出处：《重庆医学》2017年36期 　论文类型：期刊论文

【摘要】：目的探讨过氧化物酶体增殖物受体γ共激活因子-1α(PGC-1α)对肝缺血再灌注损伤的保护作用。方法构建大鼠肝缺血再灌注损伤模型,恢复灌注后12h,以蛋白免疫印迹(Western blot)检测PGC-1α表达情况,并检测肝脏活性氧、三磷酸腺苷(ATP)水平及血丙氨酸氨基转移酶(ALT)活性变化,评估肝功能情况。另一方面,构建PGC-1α慢病毒过表达载体,并在大鼠缺血再灌注前转染,于缺血再灌注后,再以Western blot检测PGC-1α表达,肝脏活性氧、ATP水平和血肝酶活性变化,评估PGC-1α表达对肝缺血再灌注损伤的保护作用。结果缺血再灌注肝脏PGC-1α表达较假手术组及对照组(未手术)明显降低(均P0.05),同时肝脏活性氧族水平及ALT活性显著升高,而肝ATP产生减少(均P0.05)。PGC-1α慢病毒过表达载体转染显著上调缺血再灌注肝脏PGC-1α表达,同时肝脏活性氧族水平及ALT活性较未转染组显著降低,而肝ATP产生增加(均P0.05)。结论 PGC-1α表达有利于保护肝缺血再灌注损伤。
[Abstract]:Objective to investigate the protective effect of peroxisome proliferator receptor 纬 co-activator 1 伪 (PGC-1 伪) on hepatic ischemia-reperfusion injury in rats. The expression of PGC-1 伪 and reactive oxygen species (Ros) in liver were detected by Western blot 12 hours after reperfusion. Adenosine triphosphate (ATP) level and alanine aminotransferase (alt) activity were measured to evaluate liver function. On the other hand, PGC-1 伪 lentivirus overexpression vector was constructed. The expression of PGC-1 伪, the level of reactive oxygen species (Ros) in liver and the activity of liver enzyme were detected by Western blot after ischemia-reperfusion in rats. To evaluate the protective effect of PGC-1 伪 expression on hepatic ischemia-reperfusion injury. Results the expression of PGC-1 伪 in ischemia-reperfusion liver was significantly lower than that in sham operation group and control group (all P 0.05). At the same time, the level of reactive oxygen species and the activity of ALT in liver were significantly increased. However, liver ATP production was decreased (all P0.05N. PGC-1 伪 lentivirus overexpression vector transfection significantly upregulated liver PGC-1 伪 expression after ischemia reperfusion. At the same time, the level of reactive oxygen species and the activity of ALT in the liver were significantly lower than those in the untransfected group, while the liver ATP production was increased (P 0.05). Conclusion the expression of PGC-1 伪 is beneficial to the protection of liver ischemia-reperfusion injury.
【作者单位】：陆军军医大学西南医院肝胆外科;
【分类号】：R657.3
【正文快照】： 肝缺血再灌注损伤是肝移植及肝切除术后急性肝功能损伤的主要原因[1-2]。研究发现肝缺血再灌注后,肝脏线粒体融合蛋白2(Mfn2)表达显著降低,且与肝功能损害程度相关。进一步研究表明Mfn2作为下游靶蛋白,受过氧化物酶体增殖物受体γ共激活因子-1α(peroxisome proliferator-acti

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