肝囊型包虫病患者手术前后血清IL-10表达及其临床意义

发布时间：2018-01-30 03:57

本文关键词： 肝囊性包虫病 IL-10 酶联免疫吸附分析法　出处：《青海大学》2015年硕士论文　论文类型：学位论文

【摘要】：目的:检测肝囊型包虫病患者手术前及术后一段时间血清IL-10水平动态变化,探讨IL-10的表达在肝囊性包虫病的发生、发展及预后中的作用及其临床意义。方法:研究30例已确诊为囊型肝包虫病且需要手术的患者,分别分为手术前、手术后1周、手术后1月、手术后3月组,并以当地30例健康人做为对照组,分别静脉抽血,离心取血清,采用酶联免疫吸附分析法(ELISA)检测样本中IL-10表达水平。结果:肝囊型包虫组血清中IL-10表达水平高于正常组,差异有统计学意义(P0.05),术后7天组IL-10表达水平高于术前组,术后1月组IL-10表达水平下降低于术前组但高于正常组,术后3月组IL-10表达水平下降趋于接近正常组,各组之间的比较差异均有统计学意义(P0.05)。结论:白细胞介素10(Interleukin 10,IL-10)是人体中一个重要的免疫调节因子,在肝囊型包虫患者中的表达水平高于健康人,随着包虫病灶切除后,可能由于手术炎症的刺激会导致IL-10的表达水平会呈短时间的上升,但随着术后虫体抗原刺激的消失,IL-10的表达水平逐渐下降并趋于正常。
[Abstract]:Objective: to detect the dynamic changes of serum IL-10 levels before and after operation in patients with hepatic cystic hydatid disease and to explore the expression of IL-10 in the occurrence of hepatic cystic hydatid disease. Methods: thirty patients with hepatic hydatid disease who had been diagnosed as cystic hepatic hydatid disease and needed surgery were divided into three groups: one week before operation, one week after operation, January after operation and March after operation. 30 healthy people were used as the control group, the blood samples were drawn by vein, and the serum was collected by centrifugation. Elisa was used to detect the expression of IL-10 in the samples. Results: the expression of IL-10 in serum of hepatic cyst hydatid group was higher than that of normal group. The difference was statistically significant (P 0.05). On the 7th day after operation, the expression of IL-10 in the group was higher than that in the group before operation, and the expression of IL-10 in the group of January after operation was lower than that in the group before operation but higher than that in the normal group. On March, the expression of IL-10 decreased to close to the normal group. The difference between each group was statistically significant (P 0.05). Conclusion: interleukin 10 is Interleukin 10. IL-10 is an important immunomodulatory factor in human body. The expression level of IL-10 in patients with hepatic cystic hydatid is higher than that in healthy people, with the removal of hydatid foci. It is possible that the level of IL-10 expression will increase in a short period of time due to the stimulation of inflammation, but the expression of IL-10 decreases gradually and tends to normal with the disappearance of the stimulation of worm body antigen after operation.
【学位授予单位】：青海大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R657.3

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