脊柱结核不同病灶组织总RNA量的比较研究

发布时间：2018-02-01 15:23

本文关键词： 脊柱结核不同病灶组织总RNA　出处：《宁夏医科大学》2015年硕士论文　论文类型：学位论文

【摘要】：研究目的:观察脊柱结核不同病灶组织中总RNA的产量和质量。实验研究方法:30例脊柱结核患者均行彻底清除病灶术,术后用无菌的EP管收集患者的脓液、死骨、肉芽、病灶壁、硬化骨(所有组织标本均用无菌剪刀、咬骨剪等器械剪碎),立即选取各种组织标本50mg液氮冷冻后用研钵彻底研碎,加入Trizol试剂1ml进行总RNA的提取;再选取30例冰冻一年的脊柱结核不同病灶组织标本,也用同样的方法进行总RNA的提取,应用ND-100分光光度仪检测不同病灶组织中的总RNA的产量和质量,同时用1%的琼脂糖凝胶电泳分别检测所提取总RNA的完整性。实验结果:新鲜的脊柱结核不同病灶组织标本中,脓液组织中含有的总RNA量大且质量好,其次为肉芽组织,含有总RNA量最少的为硬化骨,所有提取的总RNA经琼脂糖凝胶电泳以后条带清晰完整且28S:18S的亮度为2:1。冰冻一年的不同病灶组织标本中脓液组织的总RNA量最大且各种组织的总RNA经琼脂糖凝胶电泳后完整且28S为18S亮度的2倍,部分组织有少量的5S条带。实验结论:新鲜的脊柱结核不同病灶组织标本中脓液中含有的总RNA量大且质量好,而且完整性良好,完全可以满足后续的实验要求;冰冻一年的病灶组织标本有少量的RNA发生降解;与新鲜组织标本中总RNA量相比,冰冻一年的病灶组织标本同样可以用于后续的实验研究。
[Abstract]:Objective: to observe the yield and quality of total RNA in different lesions of spinal tuberculosis. The sepsis, dead bone, granulation, lesion wall and sclerosed bone were collected with sterile EP tube after operation (all tissue specimens were shredded with sterile scissors, bone biting scissors and other instruments). All kinds of tissue samples were frozen with 50mg liquid nitrogen and then ground thoroughly with mortar. The total RNA was extracted by adding 1ml of Trizol reagent. In addition, 30 specimens of different lesions of spinal tuberculosis were collected from 30 cases of frozen spinal tuberculosis for one year, and the total RNA was extracted by the same method. The output and quality of total RNA in different lesion tissues were detected by ND-100 spectrophotometer. At the same time, the integrity of total RNA was detected by 1% agarose gel electrophoresis. Results: the total RNA in the tissue of fresh spinal tuberculosis was high and the quality was good. The next was granulation tissue, and the sclerosing bone had the least total RNA. All the total RNA extracted by agarose gel electrophoresis were clear and complete, and the brightness of 28s: 18s was 2: 1. 1. The total RNA content of the pus tissue was the largest and the total RNA of various tissues was complete after agarose gel electrophoresis, and 28s was twice as bright as 18s. There were a few 5s bands in some tissues. Conclusion: the total RNA in the abscess of fresh spinal tuberculosis tissues is large, good quality and good integrity. It can completely meet the requirements of subsequent experiments. A small amount of RNA was degraded in frozen tissue samples for one year. Compared with the total amount of RNA in fresh tissue samples, frozen tissue samples for one year can also be used in subsequent experimental studies.
【学位授予单位】：宁夏医科大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R687.3

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