创伤性脑损伤大鼠ERK-MAPK信号传导途径的动态变化及意义

发布时间：2018-02-05 19:18

本文关键词： 创伤性脑损伤丝裂原活化蛋白激酶信号传导　出处：《中国老年学杂志》2017年17期 　论文类型：期刊论文

【摘要】：目的探讨大鼠创伤性脑损伤(TBI)急性期脑皮质神经细胞细胞外调节蛋白激酶(ERK)-丝裂原活化蛋白激酶(MAPK)信号通路的动态变化及意义。方法将实验大鼠通过外力导致TBI,脑损伤后皮层下微量注射ERK-MAPK信号传导抑制剂。通过Western印迹检测p ERK在大脑各部位的表达水平,末端脱氧核苷酸转移酸(TUNEL)荧光法检测神经细胞凋亡。结果 Western印迹法检测脑损伤后1、6 h,1、3、5 d时p ERK表达,发现TBI组p ERK在伤后1 h起迅速升高,3 d时为最高,损伤后5 d仍高于对照组。TBI+SCH772984组p ERK水平较同时间段TBI组减少(P0.01)。TUNEL法发现TBI+SCH772984组损伤处检测凋亡细胞明显少于对照组(P0.05),其中TBI+SCH 50mg组降低程度最明显(P0.01)。结论TBI后出现了高水平的ERK-MAPK信号通路激活,用抑制剂SCH772984对ERK-MAPK信号通路进行抑制,可以保护神经细胞,避免凋亡。
[Abstract]:Objective to investigate the rats with traumatic brain injury (TBI) in acute stage of cerebral cortex nerve cell extracellular regulated protein kinase (ERK) - mitogen activated protein kinase (MAPK) signaling pathway changes and significance. Methods rats by external force TBI, brain injury after subcortical micro injection of ERK-MAPK through signal transduction inhibitor. Western blot detection of P ERK in different parts of the brain the expression level of terminal deoxynucleotidyl transferase acid (TUNEL) nerve cell apoptosis detected by fluorescent Western blotting. Results after brain injury, 1,6 h, 1,3,5 D P ERK TBI P expression, the ERK group at 1 h after injury has increased rapidly, 3 D is the highest, 5 d after injury is still higher than that of the control P ERK group.TBI+SCH772984 group compared with the same period in TBI group decreased (P0.01) TBI+SCH772984 injury group apoptosis detection was significantly less than the control group (P0.05),.TUNEL was found in the TBI+SCH group 50mg The lowest level was the most obvious (P0.01). Conclusion a high level of ERK-MAPK signaling pathway is activated after TBI. Inhibition of ERK-MAPK signaling pathway by inhibitor SCH772984 can protect neurons and avoid apoptosis.

【作者单位】：吉林大学第一医院;长春市中医院;
【基金】：北京协和医学基金会“睿意基金”急诊医学研究专项基金(No.R2015024)
【分类号】：R651.15

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