缺氧培养下M2型巨噬细胞上清液及杜仲总黄酮对成骨细胞生物行为学的影响

发布时间：2018-02-26 23:14

本文关键词： 缺氧巨噬细胞杜仲科黄酮类组织工程组织构建骨细胞 M型巨噬细胞杜仲总黄酮成骨细胞细胞增殖成骨分化矿化作用　出处：《中国组织工程研究》2017年12期 　论文类型：期刊论文

【摘要】：背景:含成骨细胞成分的组织工程材料在骨科领域广泛应用,如何提高成骨细胞对缺氧条件的耐受能力,改善缺氧条件下成骨细胞的生物学行为,是组织工程的重要研究目标。目的:探究M2型巨噬细胞上清液和杜仲总黄酮对缺氧环境培养下的成骨细胞生物学行为的影响,为组织工程研究提供基础。方法:复苏MC3T3-E1细胞,分离、诱导及培养M2型巨噬细胞提取上清液,分别为空白对照组、M2型巨噬细胞上清液组、杜仲总黄酮组及M2型巨噬细胞上清液联合杜仲总黄酮组。空白对照组细胞不进行干预。M2型巨噬细胞上清液组、杜仲总黄酮组及M2型巨噬细胞上清液联合杜仲总黄酮组分别加入M2型巨噬细胞上清液和/或100 mg/L杜仲总黄酮。结果与结论:M2型巨噬细胞上清液组、杜仲总黄酮组及M2型巨噬细胞上清液联合杜仲总黄酮组细胞活性明显高于对照组,且M2型巨噬细胞上清液联合杜仲总黄酮组S期和G_2/M期细胞百分比显著高于对照组。M2型巨噬细胞上清液联合杜仲总黄酮组Runx2蛋白、Ⅰ型胶原蛋白、碱性磷酸酶相对mRNA水平、碱性磷酸酶相对活性以及矿化钙离子相对浓度均显著高于其他各组。提示M2型巨噬细胞上清液联合杜仲总黄酮能够促进缺氧条件下成骨细胞的增殖和分化,维持成骨细胞正常矿化功能和成骨作用,提高成骨细胞对缺氧条件的耐受能力。
[Abstract]:Background: tissue engineering materials containing osteoblasts are widely used in orthopedics. How to improve the tolerance of osteoblasts to hypoxia and improve the biological behavior of osteoblasts under hypoxia. Objective: to investigate the effects of M2 macrophage supernatant and Eucommia ulmoides flavonoids on the biological behavior of osteoblasts cultured in anoxic environment. Methods: MC3T3-E1 cells were resuscitated. The supernatant of M _ 2 macrophage was isolated, induced and cultured, and the supernatant of M _ 2 macrophage was isolated from the blank control group. The total flavonoids of Eucommia ulmoides ulmoides and the supernatant of M 2 macrophages combined with total flavonoids of Eucommia ulmoides ulmoides. Total flavonoids of Eucommia ulmoides ulmoides and supernatants of M 2 macrophages and / or total flavonoids of Eucommia ulmoides ulmoides for 100 mg/L were added into the supernatant of M 2 macrophages and / or total flavonoids of Eucommia ulmoides ulmoides respectively. The activity of total flavonoids of Eucommia ulmoides ulmoides and M 2 macrophage supernatant combined with total flavonoids of Eucommia ulmoides ulmoides were significantly higher than that of control group. The percentages of M 2 macrophage supernatant combined with total flavonoids of Eucommia ulmoides ulmoides in S phase and G 2 / M phase were significantly higher than those in control group. The levels of Runx2 protein, collagen type I, alkaline phosphatase relative mRNA were significantly higher in M 2 macrophage supernatant combined with total flavonoids of Eucommia ulmoides. The relative activity of alkaline phosphatase and the relative concentration of mineralized calcium were significantly higher than those of other groups, suggesting that the supernatant of M2 macrophage combined with the total flavonoids of Eucommia ulmoides could promote the proliferation and differentiation of osteoblasts under hypoxia. To maintain the normal mineralization function and osteogenic action of osteoblasts, and to improve the tolerance of osteoblasts to hypoxia conditions.
【作者单位】：南京中医药大学;南京中医药大学无锡附属医院骨科;
【基金】：江苏省“333”工程资助项目(BRA2012031)~~
【分类号】：R318.08;R68

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