布托啡诺对脓毒症大鼠心肌细胞的影响

发布时间：2018-02-26 23:16

  本文关键词： 布托啡诺 盲肠接扎法 脓毒血症 心肌标志物　出处：《江西医药》2017年11期 　论文类型：期刊论文

【摘要】：目的探讨布托啡诺在脓毒血症中对心肌细胞的影响及其可能机制,为临床寻找脓毒血症中心肌损伤保护的可行方法提供依据。方法健康雄性SD大鼠(由南昌大学试验动物科学部提供)81只,12-15周龄,随机分为3组。采用随机、阳性对照的试验设计方法,本临床研究的试验组和对照组,按1:1:1的比例分配。按照统计学的最低要求,每组有效病例27例,分假手术组(A)、脓毒症组(B)、布托啡诺+脓毒症组(C),共81例,A、B、C组,A组缓慢静脉推注生理盐水5ml/kg,B组和C组经股静脉缓慢静脉推注布托啡诺50μg/kg。B组和C组采用盲肠结扎穿孔法制备脓毒症模型。术前进食12h,自由饮水,腹腔注射2%戊巴比妥钠50mg/kg麻醉下,仰卧位固定,腹部备皮消毒,沿腹部正中线纵行作一长约1.5cm切口,分离肠细膜及盲肠,轻轻挤压升结肠,使肠内容物充满盲肠,以3.0丝线于回盲部下方环形结扎盲肠根部。用20G穿刺针贯穿已结扎端2次,自穿刺孔挤出肠内容物入腹腔,放置一长约2cm橡皮引流条,防止针孔闭合,然后将肠管还纳腹腔,逐层缝合腹部切口,碘伏消毒切口。A组仅暴露盲肠;选择24h为时间观察点,模型复制后24h经腹主动脉采血3ml,离心后取上清液-7℃保存备用,测定血清CK-MB及c Tn I,采用酶联免疫吸附法(EI ISA)(试剂盒购自美国雅培公司)。其余心肌组织匀浆后取上清液,用放射免疫法测定ET-1(试剂盒购自上海研域化学试剂有限公司),采用ELISA法检测TN-a(试剂盒由上海信然生物技术有限公司提供)。结果脓毒血症组与假手术组的CK-MB、c Tn I浓度比较,其显著升高(P0.01),差异有统计学意义,布托啡诺+脓毒症组与脓毒血症组CK-MB、c Tn I比较亦具有统计学意义(P0.05)。脓毒血症组TNF-a和ET-1比假手术组要明显升高,有极其明显的显著性差异(P值均0.01)。与脓毒血症组相比,布托啡诺+脓毒症组的TNF-a和ET-1明显下降,差异也具有统计学意义(P值均0.05)。结论布托啡诺注射液能减轻脓毒症大鼠的心肌损伤。
[Abstract]:Objective to investigate the effect of butorphanol on cardiomyocytes in sepsis and its possible mechanism. Methods healthy male Sprague-Dawley rats (81 male Sprague-Dawley rats aged 12-15 weeks provided by the Department of Experimental Animal Science of Nanchang University) were randomly divided into 3 groups. The design method of the positive control trial, the experimental group and the control group in this clinical study were divided according to the ratio of 1: 1 to 1: 1. According to the minimum statistical requirements, 27 effective cases were found in each group. The patients were divided into sham-operated group, septic group, butorphanol sepsis group and butorphanol sepsis group. 81 patients were treated with cecal ligation and perforation by cecal ligation in group A (5 ml / kg) via femoral vein and group C (group C) by slow intravenous injection of butorphanol (50 渭 g / kg 路kg 路B) via femoral vein. The model of sepsis was established by preoperation. Eating for 12 hours before operation and drinking freely. Under intraperitoneal injection of 2% pentobarbital sodium 50 mg / kg anesthesia, supine position was fixed, abdominal skin was disinfected, a 1.5 cm long incision was made along the median line of abdomen, the thin film of intestine and cecum were separated, and the ascending colon was pressed gently, so that the intestinal contents were filled with cecum. The root of the caecum was ligated with 3.0 silk thread in a circular way under the ileocecal part. The intestinal contents were extruded from the puncture hole into the abdominal cavity twice with a 20G puncture needle, and a rubber drainage strip of about 2 cm was placed to prevent the closure of the pinhole, and then the intestinal tube was returned to the abdominal cavity. The abdominal incision was sutured layer by layer, and the caecum was only exposed in group A. 24 hours after the model was duplicated, the blood was collected from abdominal aorta at 3ml, then the supernatant was taken for preservation at -7 鈩，

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