减重步行训练对不完全脊髓损伤大鼠脚桥核可塑性影响的研究

发布时间：2018-03-02 02:36

本文关键词： 不完全性脊髓损伤减重步行训练脚桥核型囊泡谷氨酸转运体　出处：《中国康复医学杂志》2017年06期 　论文类型：期刊论文

【摘要】：目的:探索减重步行训练对不完全脊髓损伤大鼠脚桥核可塑性的影响。方法:将雌性SD大鼠24只分为减重步行训练组、未训练组和假手术组,每组8只,用美国NYU脊髓冲击损伤仪制作大鼠T10脊髓不完全损伤模型。采用PET-CT、免疫荧光染色及BBB评分观察减重步行训练对不完全脊髓损伤(iSCI)大鼠中脑运动区(MLR)脚桥核可塑性变化和后肢运动功能的改善。结果:减重步行训练组大鼠的后肢运动功能BBB评分在造模后4周、7周两个时间点的分值均较未训练组高(P0.05)。假手术组大鼠在造模后1周、4周、7周3个时间点的BBB评分值均明显高于训练组和未训练组(P0.01)。虽然训练组18F-氟脱氧葡萄糖(18F-FDG)的标准摄取值(SUV)的平均值较未训练组增高,而且假手术组的SUV值最高,但三组SUV值的差异不具有显著性意义(P0.05)。神经元特异性核抗原(NeuN)免疫荧光染色检测显示训练组、未训练组、假手术组三组间大鼠脚桥核的神经元数量差异无显著性意义(P0.05),而脚桥核2型囊泡谷氨酸转运体(VGLUT2)的积分光密度值(IOD)比较,假手术组IOD表达最高,训练组较未训练组明显增强(P0.01)。结论:减重步行训练能够明显提高iSCI大鼠后肢运动功能及其脚桥核中谷氨酸能信号传递,而且iSCI后大鼠中脑脚桥核的整体代谢及神经元数量无明显改变。因此认为脚桥核的谷氨酸表达增强是减重步行训练使iSCI大鼠后肢步行功能改善的原因之一。减重步行训练能使脚桥核发生神经递质表达层面的可塑性变化。
[Abstract]:Objective: to explore the effect of weight-loss walking training on the plasticity of pontine nucleus in rats with incomplete spinal cord injury. Methods: Twenty-four female Sprague-Dawley rats were divided into weight-loss walking training group, untrained group and sham-operated group with 8 rats in each group. The model of incomplete injury of T10 spinal cord in rats was made by NYU spinal cord impact injury instrument. The plasticity of the pontine nucleus of the foot of the midbrain motor area in rats with incomplete spinal cord injury was observed by PET-CT, immunofluorescence staining and BBB score. Results: the BBB score of hind limb motor function in the weight-loss walking training group was higher than that in the untrained group at 4 weeks and 7 weeks after modeling (P 0.05). The BBB scores of the three time points at 4 weeks and 7 weeks were significantly higher than those of the training group and the untrained group (P 0.01), although the standard uptake of 18F-FDG in the training group was higher than that in the untrained group, although the average value of 18F-FDG in the training group was higher than that in the untrained group. The SUV value of the sham operation group was the highest, but the difference of SUV value among the three groups was not significant (P 0.05). The detection of neuron specific nuclear antigen (Neun) immunofluorescence staining showed that the training group, the untrained group, the training group, and the untrained group, There was no significant difference in the number of neurons in the pontine nucleus between the three groups of sham-operated rats, but the expression of IOD was the highest in the sham operation group compared with the integral optical density (IOD) of type 2 vesicular glutamate transporter (VGLUT2). Conclusion: weight-loss walking training can significantly improve the motor function of hind limbs and glutaminergic signal transduction in the pontine nucleus of the feet of iSCI rats. There was no significant change in the metabolism and the number of neurons in the pontine nucleus after iSCI. Therefore, it is considered that the increase of glutamate expression in the pontine nucleus of the foot is one of the reasons for the improvement of the walking function of the hind limbs of iSCI rats after weight-loss walking training. Walking training can change the plasticity of neurotransmitter expression in the nucleus of the foot.
【作者单位】：南方医科大学第二临床医学院;陆军总医院骨科研究所;解放军医学院;空军总医院康复科;
【基金】：国家自然科学基金应急管理项目(81650013);国家自然科学基金面上项目(81171862)
【分类号】：R651.2

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