带部分松质骨小牛皮质骨复合骨髓间充质干细胞植入兔体内成骨及骨形态发生蛋白2的表达

发布时间：2018-03-04 08:26

本文选题：干细胞　切入点：骨形态发生蛋白质类　出处：《中国组织工程研究》2017年17期 　论文类型：期刊论文

【摘要】：背景:前期研究已证实兔骨髓间充质干细胞在体外成骨诱导培养下可定向成骨分化,并能够稳定表达成骨细胞的特异性表型,具有成骨能力;且已证实带部分松质骨的小牛皮质骨支架材料具有良好的生物相容性和降解性,可以作为骨髓间充质干细胞的载体材料。目的:在前期研究基础上,根据骨组织工程学的基本原理,将骨髓间充质干细胞与小牛异种骨复合,植入新西兰大白兔髂骨内观察其成骨情况,为构建理想的初步组织工程骨工程化产品提供理论依据。方法:分别将兔骨髓间充质干细胞成骨诱导后复合的带部分松质骨小牛皮质骨(组织工程骨组)、单纯小牛异种骨(异种骨组)、自体髂骨(自体髂骨组)随机植入兔髂骨。观察各组植入物的表面变化和周围组织反应,植入后4,8,12,24周摄X射线片观察成骨情况,取材后利用免疫组织化学染色观察骨形态发生蛋白2的表达情况。结果与结论:异种骨植入兔髂骨后,伤口生长良好,没有全身和局部的炎症和毒性反应。X射线片显示组织工程骨组在术后24周时与宿主骨结合部基本愈合,而异种骨组术后24周与宿主骨愈合不满意。苏木精-伊红染色可观察到软骨化骨过程,免疫组织化学染色显示各时间点骨形态发生蛋白2染色阳性。体内实验结果表明兔骨髓间充质干细胞复合带部分松质骨的小牛皮质骨支架材料经体外诱导后具有在新西兰兔体内构建组织工程骨的能力。
[Abstract]:Background: previous studies have confirmed that rabbit bone marrow mesenchymal stem cells (BMSCs) can differentiate into directional osteoblasts under osteogenic induction culture in vitro, and can stably express the specific phenotype of osteoblasts and have osteogenic ability. It has been proved that the scaffold material with partial cancellous bone has good biocompatibility and biodegradability, and can be used as the carrier material of bone marrow mesenchymal stem cells. According to the basic principle of bone tissue engineering, bone marrow mesenchymal stem cells (BMSCs) were implanted into iliac bone of New Zealand white rabbits to observe their osteogenesis. Methods: rabbit bone marrow mesenchymal stem cells (BMSCs) were used to induce osteogenesis of calf cortical bone with partial cancellous bone (tissue engineering bone group, simple and small). Bovine xenogeneic bone (allograft group) and autogenous iliac bone group (autologous iliac bone group) were randomly implanted into rabbit iliac bone. After implantation, bone morphogenetic protein-2 (BMP 2) expression was observed by immunohistochemical staining. Results and conclusion: after implantation of xenogenic bone into iliac bone of rabbits, the wound grew well, and the bone formation was observed by X-ray film at 24 weeks after implantation, and the expression of bone morphogenetic protein-2 was observed by immunohistochemical staining. There was no systemic and local inflammatory and toxic reaction. X-ray showed that the tissue engineered bone group basically healed with the host bone at 24 weeks after operation. However, 24 weeks after operation in the xenogeneic bone group, the healing of the host bone was not satisfactory. Hematoxylin and eosin staining could observe the process of osteochondrosis. Immunohistochemical staining showed that bone morphogenetic protein-2 staining was positive at different time points. The results of in vivo experiments showed that rabbit bone marrow mesenchymal stem cells combined with calf-skin bone scaffold with partial cancellous bone had a positive effect on the expression of bone morphogenetic protein-2 in vitro. Ability to construct tissue engineered bone in New Zealand rabbits.
【作者单位】：深圳市蛇口人民医院;中南大学湘雅二医院;
【基金】：湖南省科技计划项目(2013FJ6001) 深圳市科创委基金(20150305190447)~~
【分类号】：R318.08;R68

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