小剂量雌激素肌肉注射联合骨髓间充质干细胞移植修复脊髓损伤

发布时间：2018-03-04 08:27

本文选题：间质干细胞　切入点：脊髓损伤　出处：《中国组织工程研究》2017年25期 　论文类型：期刊论文

【摘要】：背景:多种手段联合应用可针对不同靶点进行有效干预,发挥叠加或协同作用,已成为当前脊髓损伤治疗的新思路。目的:探讨骨髓间充质干细胞移植联合应用小剂量雌激素对大鼠脊髓损伤后神经功能恢复及炎症反应的影响。方法:将70只雄性SD大鼠随机分5组,假手术组(n=10)仅暴露脊髓,不做损伤处理;模型组、雌激素组、干细胞组、联合组(每组15只)采用改良的Allen’s法建立脊髓损伤模型。干细胞组、联合组造模后立即在损伤部位移植骨髓间充质干细胞;雌激素组、联合组造模后第1,24小时肌肉注射17β-雌二醇。造模后第1,3,5,7天,进行BBB行为学评价;造模后6,12,24,72 h,应用ELISA法测定脊髓匀浆组织白细胞介素1β、肿瘤坏死因子α水平,TUNEL染色观察脊髓组织切片神经细胞凋亡情况。结果与结论:(1)与假手术组比较,模型组造模后不同时间点的BBB行为学评分下降(P0.05);与模型组比较,干细胞、雌激素组、联合组造模后5,7 d的BBB行为学评分升高(P0.05),且联合组高于干细胞、雌激素组(P0.05);(2)与假手术组比较,模型组造模后6,12,24 h的白细胞介素1β、肿瘤坏死因子α水平升高(P0.05);与模型组比较,干细胞、雌激素组、联合组造模后12,24 h的白细胞介素1β、肿瘤坏死因子α水平降低(P0.05),且联合组低于干细胞、雌激素组(P0.05);(3)造模后72 h,模型组神经细胞凋亡率高于假手术组,干细胞、雌激素组、联合组低于模型组(P0.05),且以联合组最低(P0.05);(4)结果表明在脊髓损伤中,雌激素与骨髓间充质干细胞联合应用具有协同作用,能更有效抑制神经细胞凋亡,促进脊髓功能恢复。
[Abstract]:Background: the combined use of multiple means can effectively intervene against different targets and play a superimposed or synergistic role. It has become a new idea to treat spinal cord injury. Objective: to investigate the effect of bone marrow mesenchymal stem cell transplantation combined with low dose estrogen on the recovery of nerve function and inflammatory response after spinal cord injury in 70 rats. Male SD rats were randomly divided into 5 groups. Model group, estrogen group, stem cell group, combined group (15 rats in each group) were used to establish spinal cord injury model with modified Allen's method. Bone marrow mesenchymal stem cells (BMSCs) were transplanted immediately after modeling in the combined group, and 17 尾 -estradiol was injected intramuscularly in the estrogen group at 1h after modeling. The behavior of BBB was evaluated by BBB. The expression of IL-1 尾 and TNF- 伪 levels in spinal cord homogenate tissue were determined by ELISA method and Tunel staining was used to observe the apoptosis of neurons in spinal cord tissue slices. Results and conclusion: 1) compared with sham operation group, the expression of IL-1 尾 and TNF- 伪 in spinal cord homogenate tissue were determined by ELISA method. Compared with the model group, the BBB behavioral score of stem cell, estrogen group and combination group increased P0.05 at 7 days after modeling, and higher in the combined group than that in the stem cell group, and compared with the model group at different time points after the model making, the BBB behavioral score of the model group was decreased at different time points (P0.05), and that of the combined group was higher than that of the stem cell group. Compared with sham-operated group, the levels of IL-1 尾 and TNF- 伪 in model group increased at 1224 h after modeling, compared with model group, compared with model group, stem cells and estrogen group. The levels of IL-1 尾 and TNF- 伪 in the combined group were significantly lower than those in the control group (P 0.05) and the apoptotic rate of neurons in the model group was higher than that in the sham operation group, stem cell group and estrogen group at 72 h after the establishment of the model. The results showed that the combination of estrogen and bone marrow mesenchymal stem cells had synergistic effect on spinal cord injury, which could effectively inhibit neuronal apoptosis and promote the recovery of spinal cord function.
【作者单位】：河北北方学院;河北北方学院附属第一医院;
【基金】：河北省卫生厅2011年医学科学研究重点项目计划(20110176):BMSC体外分化移植治疗脊髓损伤临床与基础研究~~
【分类号】：R651.2

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