上皮性钙通道TRPV6对乳腺分化发育的影响

发布时间：2018-03-09 07:16

本文选题：乳腺　切入点：细胞分化　出处：《南开大学学报(自然科学版)》2017年05期 　论文类型：期刊论文

【摘要】：为了研究上皮性钙通道TRPV6(transient receptor potential vanilloid 6)对乳腺分化发育的影响,本实验首先收集了发育不同时期的小鼠乳腺样本,共11个时间点,包括青春前期、青春期、孕期、泌乳期以及退化期,通过免疫荧光染色的方法观察了TRPV6在乳腺发育过程中的表达变化.其次,利用3维培养技术、Real-time PCR技术以及免疫荧光技术探究了TRPV6对正常乳腺上皮细胞MCF10A分化的影响.结果表明,在乳腺发育的不同阶段,TRPV6的表达水平具有明显差异,且在泌乳期达到最高;敲减TRPV6可以显著抑制MCF10A乳腺微球的形成,表现在成球数量的减少和微球平均直径的下降;在RNA和蛋白水平的检测结果显示TRPV6的敲减影响多个细胞分化相关分子的表达.上述结果提示TRPV6在乳腺发育过程中可能发挥重要作用.
[Abstract]:In order to study the effect of TRPV6(transient receptor potential vanilloid 6 on the differentiation and development of mammary glands, we collected breast samples from mice at different stages of development, including prepubertal, puberty and pregnancy. The expression of TRPV6 in breast development was observed by immunofluorescence staining during lactation and degenerative stage. Secondly, The effects of TRPV6 on the differentiation of MCF10A in normal breast epithelial cells were studied by means of real-time PCR technique and immunofluorescence technique. The results showed that the expression level of TRPV6 was significantly different in different stages of breast development. Knockout TRPV6 could significantly inhibit the formation of MCF10A mammary microspheres, which was shown by the decrease of the number of balls and the decrease of average diameter of microspheres. The results of RNA and protein level showed that knockout of TRPV6 affected the expression of several cell differentiation related molecules. These results suggested that TRPV6 may play an important role in breast development.
【作者单位】：南开大学生命科学学院;
【基金】：111基地经费支持(B08011) 药物化学生物学国家重点实验室(南开大学)开放基金
【分类号】：R737.9

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