RECK与MMP-9在人骨肉瘤组织中的表达及相关性分析

发布时间：2018-03-12 09:20

本文选题：伴有kazal域的富含半胱氨酸的逆转诱导蛋白　切入点：基质金属蛋白酶　出处：《中国医药导报》2016年33期 　论文类型：期刊论文

【摘要】：目的探讨伴有kazal域的富含半胱氨酸的逆转诱导蛋白(RECK)与基质金属蛋白酶9(MMP-9)在人骨肉瘤组织中的表达及相关性。方法选取唐山市人民医院2001年1月~2010年12月临床及病理资料完整存档的人骨肉瘤组织标本34例为观察组,另取临床及病理资料完整存档的骨软骨瘤组织标本34例为对照组,采用免疫组织化学法检测RECK和MMP-9,采用定量PCR法检测RECK和MMP-9的mRNA,并分析指标相关性。结果观察组RECK阳性率(58.8%)低于对照组(91.2%),而MMP-9阳性率(64.7%)高于对照组(11.8%),差异有统计学意义(P0.05)。RECK阳性率、RECK的mRNA表达、MMP-9阳性率、MMP-9的mRNA表达在年龄、性别、病发部位方面比较,差异无统计学意义(P0.05)。RECK阳性率、RECK的mRNA表达、MMP-9阳性率、MMP-9的mRNA表达在转移、复发以及生存时间方面比较,差异有统计学意义(P0.05)。RECK与人骨肉瘤组织呈明显负相关(r=-0.561,P=0.000)。MMP-9与人骨肉瘤组织呈明显正相关(r=0.613,P=0.000)。结论 RECK可抑制MMP-9表达,发挥抑癌作用,有望成为未来诊断以及治疗人骨肉瘤的潜在靶标。
[Abstract]:Objective to investigate the expression of cysteine-rich reverse inducible protein (Reck) and matrix metalloproteinase-9 (MMP-9) in human osteosarcoma with kazal domain. Methods the clinical data of Tangshan people's Hospital from January 2001 to December 2010 were studied. 34 cases of human osteosarcoma with complete archival pathological data were treated as observation group. 34 cases of osteochondroma were collected as control group. Immunohistochemical method was used to detect RECK and MMP-9, quantitative PCR method was used to detect RECK and MMP-9, and correlation was analyzed. Results the positive rate of RECK in the observation group was 58.8% lower than that in the control group (91.2%), but the positive rate of MMP-9 was 64.7%) higher than that in the control group (the difference was statistically significant). The positive rate of mRNA expression of Reck and the positive rate of MMP-9 and mRNA expression of MMP-9 were found at age. There was no significant difference in the sex and the location of the disease. There was no significant difference in the positive rate of P0.05. Reck and the positive rate of mRNA in reck. The positive rate of MMP-9 and the mRNA expression of MMP-9 were compared in the aspects of metastasis, recurrence and survival time. There was a significant negative correlation between p0.05U 路reck and human osteosarcoma tissue. MMP-9 was positively correlated with human osteosarcoma tissue. Conclusion RECK can inhibit the expression of MMP-9 and play a role in tumor inhibition, and it is expected to be a potential target for the future diagnosis and treatment of human osteosarcoma.
【作者单位】：河北省唐山市人民医院放化五科;华北理工大学医学实验研究中心;
【基金】：河北省唐山市科学技术研究与发展指导计划项目(101302114c)
【分类号】：R738.1

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