骨关节结核外周血全血细胞TLR4表达与血清TNF-α、IL-6水平的相关性研究

发布时间：2018-03-14 07:43

本文选题：骨关节结核　切入点：Toll样受体4　出处：《右江民族医学院》2017年硕士论文　论文类型：学位论文

【摘要】：目的:检测健康人群和骨关节结核患者外周血全血细胞Toll样受体4(Toll-like receptor 4,TLR4)在mRNA水平上的相对表达量;同时检测两组受试者血清中肿瘤坏死因子-α(Tumor necrosis factor-α,TNF-α)和白细胞介素-6(Interleukin-6,IL-6)的表达情况,以初步探讨骨关节结核外周血全血细胞TLR4表达情况和意义,以及其与血清TNF-α、IL-6水平的相关性。方法:(1)采用实时荧光定量PCR(Real Time PCR)技术检测TLR4 m RNA在113名健康者和81例骨关节结核患者全血细胞中的相对表达量,比较两组间的差异。(2)应用双抗体夹心免疫酶联吸附法(Enzyme linked immunosorbent assay,ELISA)对两组受试者血清中TNF-α和IL-6的表达含量进行检测,比较两组间的表达差异。(3)分析观察组中TLR4 mRNA相对表达量与血清TNF-α、IL-6水平之间的相关性,以及血清TNF-α水平与IL-6水平的相关性。结果:(1)健康对照组TLR4 mRNA的相对表达量为0.417±0.082,骨结核结核患者TLR4 mRNA的相对表达量为0.735±0.161。两组TLR4 mRNA相对表达量之间的差异具有统计学意义(P0.01)。(2)观察组患者血清TNF-α和IL-6的表达水平均高于对照组,差异均具有统计学意义(P0.01)。(3)在观察组中,TLR4 mRNA相对表达量与血清TNF-α、IL-6水平均无显著线性相关(相关系数r=0.178,P0.05;相关系数r=0.043,P0.05);血清TNF-α水平与IL-6水平亦无明显线性相关(相关系数r=-0.042,P0.05)。结论:(1)骨关节结核患者外周血全血细胞TLR4 mRNA相对表达量显著高于健康人群,提示TLR4基因在mRNA水平上的高表达可能与骨关节结核的免疫调节存在密切联系。(2)骨关节结核患者血清TNF-α和IL-6水平明显高于健康人群,说明其可能在骨关节结核的发生发展中起着重要的作用。(3)在骨关节结核患者中,外周血全血细胞TLR4 mRNA相对表达量与血清TNF-α、IL-6水平均无显著线性相关。
[Abstract]:Objective: to detect the relative expression of Toll like receptor 4Toll-like receptor 4 (TLR4) in peripheral blood of healthy people and patients with bone and joint tuberculosis at the level of mRNA, and to detect the expression of tumor necrosis factor- 伪 Tumor necrosis factor- 伪 (TNF- 伪) and interleukin-6 Interleukin-6IL-6 (IL-6) in the serum of both groups. To explore the expression and significance of TLR4 in peripheral blood of osteoarticular tuberculosis. Methods the relative expression of TLR4 m RNA in the whole blood cells of 113 healthy subjects and 81 patients with osteoarticular tuberculosis was detected by real-time fluorescence quantitative PCR(Real Time PCR. The expression of TNF- 伪 and IL-6 in serum of the two groups was detected by double antibody sandwich immunosorbent assay (Elisa). To analyze the correlation between the relative expression of TLR4 mRNA and the serum TNF- 伪 IL-6 level in the observation group. Results the relative expression of TLR4 mRNA in healthy control group was 0.417 卤0.082, and the relative expression of TLR4 mRNA in tuberculosis of bone was 0.735 卤0.161. The difference between the two groups was statistically significant. The serum levels of TNF- 伪 and IL-6 in the observation group were higher than those in the control group. There was no significant linear correlation between the relative expression of TLR4 mRNA and serum TNF- 伪 IL-6 level in the observation group (r = 0.178, P 0.05, r = 0.043, P 0.05, r = 0.043, P 0.05, respectively), and there was no significant linear correlation between the serum TNF- 伪 level and the level of IL-6 (correlation coefficient). Conclusion the relative expression of TLR4 mRNA in peripheral blood of patients with osteoarticular tuberculosis was significantly higher than that of healthy controls. It is suggested that the high expression of TLR4 gene at mRNA level may be closely related to the immunomodulation of osteoarticular tuberculosis.) Serum TNF- 伪 and IL-6 levels in patients with osteoarticular tuberculosis are significantly higher than those in healthy controls. It is suggested that the relative expression of TLR4 mRNA in peripheral blood cells is not linearly correlated with the serum TNF- 伪 IL-6 level in patients with osteoarticular tuberculosis, which may play an important role in the occurrence and development of osteoarticular tuberculosis.
【学位授予单位】：右江民族医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R529.2

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