壳聚糖神经导管联合PRP修复面神经缺损的实验研究

发布时间：2018-03-14 10:11

本文选题：壳聚糖神经导管　切入点：面神经缺损　出处：《临床耳鼻咽喉头颈外科杂志》2017年19期 　论文类型：期刊论文

【摘要】：目的:利用壳聚糖制作可降解神经导管,探讨壳聚糖神经导管联合自体富血小板血浆(PRP)修复面神经缺损的可行性,为临床修复面神经缺损提供新方法。方法:新西兰大白兔40只,随机分4组,各10只。建立面神经上颊支缺损模型并置于神经再生导管中,分别注入等量的PRP和生理盐水。壳聚糖神经导管加PRP为A组、壳聚糖神经导管加生理盐水为B组、硅胶管加生理盐水为C组、硅胶管加PRP为D组。术后8周进行面神经大体观察、神经电生理检测、组织学观察、图像分析、评价神经再生恢复情况。结果:A、B组神经导管部分降解吸收,C、D组神经导管未降解,A、D组再生神经粗大而B、C组细小。电生理检测A、D组再生神经传导速度及波幅均优于B、C组(P0.05),A、D组肌肉动作电位潜伏期低于B、C组(P0.05),A组与D组再生神经传导速度、波幅及肌肉动作电位潜伏期差异无统计学意义(P0.05)。图像分析有髓神经纤维数目及髓鞘厚度,A、D组优于B、C组(P0.05),A组与D组比较差异无统计学意义(P0.05)。结论:壳聚糖神经导管联合PRP可作为一种新的面神经缺损修复手段,其效果较好,有望应用于临床。
[Abstract]:Objective: to study the feasibility of chitosan nerve catheter combined with autogenous platelet-rich plasma (PRP) for the repair of facial nerve defect. Methods: 40 New Zealand white rabbits were randomly divided into 4 groups, 10 in each group. The model of buccal branch defect of the facial nerve was established and placed in the nerve regeneration catheter. The same amount of PRP and saline were injected into group A. chitosan nerve conduit plus PRP was used as group A, chitosan nerve catheter and saline as group B, silicone tube and saline as C group. After 8 weeks of operation, the facial nerve gross observation, nerve electrophysiological examination, histological observation and image analysis were performed. Results the regenerated nerve was coarse in group C and small in group B, and the conduction velocity and amplitude of regenerated nerve in group A were better than that in group B. The latent period of muscle action potential in group A was lower than that in group C (P 0.05) and the conduction velocity of regenerative nerve in group D was lower than that in group B (P 0.05). There was no significant difference in amplitude and latency of muscle action potential (P 0.05). There was no significant difference in the number of myelinated nerve fibers and the thickness of myelin sheath between group A and group D. Conclusion: there is no significant difference between group A and group D in the number of myelinated nerve fibers and the thickness of myelin sheath. Conclusion: there is no significant difference in the number of myelinated nerve fibers and the thickness of myelin sheath between group A and group D. Combined with PRP can be used as a new method for repairing facial nerve defects. It is expected to be used in clinical practice.
【作者单位】：大连大学附属中山医院口腔颌面外科;大连医科大学附属大连市口腔医院牙体牙髓科;
【分类号】：R651.3

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