正中神经电刺激对脑外伤后昏迷大鼠前额叶皮质区NMDA NR1蛋白表达变化的影响

发布时间：2018-03-15 12:32

本文选题：NR1　切入点：Orexin-A　出处：《南昌大学》2016年硕士论文　论文类型：学位论文

【摘要】：目的:研究正中神经电刺激对脑外伤昏迷大鼠前额叶皮质区(prefrontal cortex,PFC)NMDA NR1受体水平的影响。方法:将144只SD大鼠随机分为空白组,假刺激组,刺激组和OX1R拮抗剂组共4组。采用经典的自由落体模型建立脑外伤昏迷模型及大鼠意识状态6级评定标准评估四组大鼠行为学变化,并于实验完成后6,12,24小时处死大鼠,分别用Western-blot及免疫组织化技术检测各组大鼠前额叶皮质组织的NMDA NR1受体表达。结果:刺激组25只大鼠(25/36)出现翻正反射,OX1R拮抗剂组17只(17/36)、假刺激组11只(11/36)出现翻正反射。Western-blot法结果显示四组NMDA NR1受体水平在6,12,24h三个时间点均呈现“OX1R拮抗剂组假刺激组空白组刺激组”递增趋势,且差异有统计学意义(P0.05),而同一组不同时间点的比较表现为:空白组及假刺激组:“6h12h24h”,差异有统计学意义(P0.05);刺激组:“6h12h24h”,差异无统计学意义(P㧐0.05);OX1R拮抗剂组:“6h24h12h”,差异有统计学意义(P0.05)。免疫组化结果显示组间比较前额叶组织NMDA NR1受体表达呈“OX1R拮抗剂组假刺激组空白组刺激组”递增趋势(P0.05),组内比较呈“6h12h24h”趋势(P㧐0.05)。结论:正中神经电刺激可作为一种治疗脑外伤后昏迷的有效促醒方法,其机制可能与前额叶皮质NMDA NR1受体水平上调有关,且Orexin-A可能在其中发挥调节作用。
[Abstract]:Objective: to study the effect of median nerve electrical stimulation on the level of NMDA NR1 receptor in prefrontal cortex of prefrontal cortex of rats with brain injury. Methods: 144 SD rats were randomly divided into blank group and sham stimulation group. The rats in the stimulation group and the OX1R antagonist group were divided into 4 groups. The model of brain trauma coma was established by the classical free-fall body model and the rats' consciousness status was assessed by the 6-grade assessment standard. The rats were killed at 61212h 24 hours after the experiment, and the rats were killed 24 hours after the experiment. The expression of NMDA NR1 receptor in prefrontal cortex of rats in each group was detected by Western-blot and immunohistochemistry respectively. Results: in the stimulation group, 25 / 36 rats had positive and positive reflex OX1R antagonist group (17 / 17 / 36) and the sham stimulation group (11 / 11 / 36) had positive and negative inversion. Western-blot analysis showed that the level of NMDA NR1 receptor in the four groups showed an increasing trend at the three time points of 6 ~ 1212 ~ 24 h, "OX1R antagonist group, sham stimulation group, blank stimulation group", and "OX1R antagonist group, sham stimulation group, blank stimulation group". The difference was statistically significant (P 0.05), but the difference between blank group and sham stimulation group was "6 h 12 h 24 h" (P 0.05), while that of stimulation group was "6 h 12 h 24 h" (P < 0.05), but there was no significant difference between control group and sham stimulation group (P < 0.05). The results of immunohistochemistry showed that the expression of NMDA NR1 receptor in prefrontal lobe tissue of OX1R antagonist group showed an increasing trend of increasing (P0.05N), while the expression of NR1 receptor in prefrontal lobe tissue showed a trend of "6h 12h 24 h" in the control group of OX1R antagonist group and "6h 12h 24 h" in the group with P0. 05% OX1R antagonist, and there was a tendency of P0. 05% expression in the prefrontal lobe tissue of the group compared with that of the control group (P 0. 05%). Conclusion: median nerve stimulation can be used as an effective method for the treatment of coma after brain injury. The mechanism may be related to the up-regulation of NMDA NR1 receptor in prefrontal cortex, and Orexin-A may play a regulatory role in it.
【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R651.15

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