骨性关节炎相关基因MCF2L可增加滑膜成纤维细胞凋亡及诱导炎症反应

发布时间：2018-03-15 12:51

本文选题：骨性关节炎　切入点：凋亡　出处：《基因组学与应用生物学》2017年06期 　论文类型：期刊论文

【摘要】：MCF2L基因突变在骨关节炎的发生与发展过程中扮演重要作用,本研究使用ENSEMBL及COMSIC数据库筛选MCF2L突变位点,构建质粒及腺病毒载体;将小鼠原代滑膜成纤维细胞分为正常对照组,野生型重组腺病毒干预的MCF2L过表达组和突变型重组腺病毒的突变组,检测细胞上清中IL-lβ、MMP-13、PGE2水平及细胞凋亡。通过数据库检测分析发现可将第11位精氨酸作为突变位点;p Yr-ads-4-MCF2L重组质粒及突变型和野生型重组腺病毒包装成功;突变组MCF2L RNA表达是对照组的1.92倍,过表达组是对照组的1.85倍;突变组及过表达组MCF2L蛋白表达水平均显著高于对照组;过表达组上清中PGE2、MMP-13、IL-lβ水平显著低于对照组(p0.05),突变组上清中PGE2、MMP-13、IL-lβ水平显著高于对照组(p0.05),且突变组上清中PGE2、MMP-13、IL-lβ水平明显高于过表达组,且差异存在统计学意义(p0.05);对照组及过表达组细胞凋亡不明显,在1%至10%之间;突变组细胞凋亡率高于对照组及过表达组,凋亡率达60%。滑膜成纤维细胞使用MCF2L基因突变重组腺病毒感染后,PGE2、MMP-13、IL-lβ水平均明显提高,细胞凋亡率明显加大。本研究对MCF2L的单核苷酸多态性对滑膜成纤维细胞的炎症因子及凋亡活性的影响进行分析,探讨骨性关节炎发病过程中MCF2L发挥的作用,探讨MCF2L基因与骨关炎的关系,为临床应用提供依据。
[Abstract]:MCF2L gene mutation plays an important role in the occurrence and development of osteoarthritis. In this study, ENSEMBL and COMSIC databases were used to screen MCF2L mutation sites, construct plasmid and adenovirus vector, and divide mouse primary synovial fibroblasts into normal control group. The MCF2L overexpression group and mutant recombinant adenovirus mutation group were induced by wild type recombinant adenovirus. The level of IL-l 尾 -MMP-13, PGE2 and apoptosis in the supernatant were detected. The recombinant plasmid of IL-l 尾 -MMP-13 PGE2 and the recombinant adenovirus containing mutant and wild type adenovirus could be successfully packaged with arginine at the 11th position as the mutation site. The expression of MCF2L RNA in mutant group was 1.92 times higher than that in control group, and that in overexpression group was 1.85 times higher than that in control group, and the expression level of MCF2L protein in mutant group and overexpression group was significantly higher than that in control group. The level of IL-l 尾 in the supernatant of the overexpression group was significantly lower than that in the control group, and the level of IL-l 尾 in the supernatant of the mutant group was significantly higher than that in the control group, and the level of IL-l 尾 in the supernatant of the mutant group was significantly higher than that in the overexpression group. The apoptosis rate of the mutant group was higher than that of the control group and overexpression group, and the apoptotic rate of the mutant group was higher than that of the control group and overexpression group. The apoptosis rate was 60%. The levels of IL-l 尾 of PGE2MMP-13 and IL-l 尾 were significantly increased in synovial fibroblasts infected with recombinant adenovirus with MCF2L gene mutation. The effect of single nucleotide polymorphism (SNP) of MCF2L on inflammatory factors and apoptosis activity of synovial fibroblasts was analyzed, and the role of MCF2L in the pathogenesis of osteoarthritis was discussed. To explore the relationship between MCF2L gene and Guguan inflammation and to provide the basis for clinical application.
【作者单位】：晋江市医院骨一科;
【基金】：泉州市科技项目(非扩髓带锁髓内钉在胫骨开放性骨折的临床应用,No.201101SH01)资助
【分类号】：R684.3

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