佐剂性关节炎模型兔成纤维样滑膜细胞的培养

发布时间：2018-03-16 00:01

本文选题：关节炎　切入点：实验性　出处：《中国组织工程研究》2017年12期 　论文类型：期刊论文

【摘要】：背景:目前研究者多采用酶消化法从类风湿性患者和佐剂性关节炎大鼠的滑膜组织中分离培养成纤维样滑膜细胞,采用组织块贴壁法体外培养佐剂性关节炎兔成纤维样滑膜细胞较少见。目的:建立佐剂性关节炎兔成纤维样滑膜细胞的培养方法并观察细胞的生物学特性。方法:采用多点多次注射佐剂的方法制备佐剂性关节炎兔动物模型,取膝关节滑膜组织,用组织块贴壁法分离培养成纤维样滑膜细胞,观察细胞形态特征。采用CCK-8法检测细胞增殖情况,并用免疫荧光法检测波形蛋白的表达。结果与结论:倒置显微镜观察原代培养的关节炎兔滑膜细胞形态均为长梭状纤维样,符合成纤维样滑膜细胞的生长特征,且表达波形蛋白的细胞达98%。说明采用组织块贴壁法成功分离培养了佐剂性关节炎兔成纤维样滑膜细胞。
[Abstract]:Background: fibroid synovial cells were isolated from synovial tissues of rheumatoid patients and adjuvant arthritis rats by enzyme digestion. Fibroid synovial cells were rarely cultured in rabbits with adjuvant arthritis by tissue mass adherent method. Objective: to establish the culture method of fibroid synovial cells in rabbits with adjuvant arthritis and to observe the biological characteristics of the cells. The rabbit model of adjuvant arthritis was established by multi-point multiple injection of adjuvant. The synovial tissue of the knee joint was taken and the fibroid synovial cells were isolated and cultured by tissue mass adherence method. The morphological characteristics of the cells were observed. The proliferation of the cells was detected by CCK-8 method. The expression of vimentin was detected by immunofluorescence. Results and conclusion: the morphology of synovial cells in primary cultured rabbit synovial cells was long fusiform, and the growth characteristics of fibroid synovial cells were observed by inverted microscope. The number of cells expressing vimentin was 98. The results showed that the fibroid synovial cells of adjuvant arthritis rabbits were successfully isolated and cultured by tissue mass adherence method.
【作者单位】：中国人民解放军武汉总医院医学实验科;中国人民解放军武汉总医院中西结合科;
【分类号】：R-332;R684.3

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