七氟醚预处理对小鼠Lewis肺癌细胞肺转移的抑制作用

发布时间：2018-03-18 08:19

本文选题：七氟醚　切入点：肺转移　出处：《临床麻醉学杂志》2017年01期 　论文类型：期刊论文

【摘要】：目的观察七氟醚预处理对小鼠Lewis肺癌细胞肺转移的影响。方法小鼠Lewis肺癌细胞接种于培养板,培养24h后,采用随机数字表法,将其随机分为四组:对照组(CC组)、1%七氟醚组(SC1组)、2%七氟醚组(SC2组)和3%七氟醚组(SC3组)。CC组不接受七氟醚处理,SC1~3组细胞分别用1%、2%、3%七氟醚处理4h,继续培养24h。采用Transwell法检测细胞侵袭能力,细胞划痕实验检测细胞迁移能力;采用ELISA法检测细胞MMP-2和MMP-9蛋白表达。32只C57BL/6小鼠随机分为四组:对照组(CM组)、1%七氟醚组(SM1组)、2%七氟醚组(SM2组)和3%七氟醚组(SM3组),每组8只。取SC1~3组七氟醚预处理后的Lewis肺癌细胞悬液0.1ml(2×107个/ml),分别给SM1~3组小鼠尾静脉注射,CM组小鼠注射等体积的CC组细胞悬液。3周后观察肺部转移灶,计算肺转移抑制率。结果 SC1~3组细胞侵袭能力和迁移能力明显低于CC组,且SC1组SC2组SC3组(P0.05);SC1~3组MMP-2和MMP-9浓度明显低于CC组,且SC1组SC2组SC3组(P0.05);SM1~3组肺转移抑制率明显高于CM组,且SM1组SM2组SM3组(P0.05)。结论七氟醚预处理小鼠Lewis肺癌细胞可抑制其在小鼠体内的肺转移,此效应可能与下调小鼠Lewis肺癌细胞MMP-2和MMP-9表达有关。
[Abstract]:Objective to observe the effect of sevoflurane pretreatment on lung metastasis of Lewis lung cancer cells in mice. They were randomly divided into four groups: control group (CC group), 1% sevoflurane group (group SC1) and 3% group of sevoflurane group (group C 3). Group CC was not treated with sevoflurane for 4 h and cultured for 24 h. The cells in group C were treated with 3% sevoflurane for 4 h. The ability of cell invasion, Cell migration ability was detected by cell scratch assay. 32 C57BL / 6 mice were randomly divided into four groups: control group (CM group), 1% sevoflurane group (SM1 group) and 3% sevoflurane group (SM3 group) with 8 rats in each group. Sevoflurane was pretreated with SC1~3. Lewis lung cancer cell suspension (0.1 ml / ml) was injected into caudal vein of SM1~3 group and CC group was injected with the same volume of CC cell suspension for 3 weeks to observe the metastatic foci of the lung. Results the cell invasion and migration ability in SC1~3 group was significantly lower than that in CC group, and the concentrations of MMP-2 and MMP-9 in SC3 group were significantly lower than those in CC group in SC2 group, and in SC1 group, SC3 group in SC1 group was significantly higher than that in CM group. Conclusion sevoflurane pretreated with sevoflurane can inhibit the lung metastasis of Lewis lung cancer cells in mice, which may be related to the down-regulation of MMP-2 and MMP-9 expression in Lewis lung cancer cells.
【作者单位】：佛山市第一人民医院麻醉科;
【分类号】：R614;R734.2

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