右美托咪定对过氧化氢引起的PC12细胞损伤的保护作用

发布时间：2018-03-19 10:25

本文选题：右美托咪定　切入点：PC细胞　出处：《中国老年学杂志》2017年22期 　论文类型：期刊论文

【摘要】：目的探讨右美托咪定对过氧化氢(H_2O_2)引起的PC12细胞损伤的保护作用。方法 PC12细胞随机分为正常对照组,H_2O_2组(200μmol/L H_2O_2),右美托咪定低、中、高浓度组(50、100、200μmol/L右美托咪定+200μmol/L H_2O_2),培养48 h,分别检测细胞活力、凋亡情况、天冬氨酸蛋白水解酶(Caspase)3、Caspase9活性、乳酸脱氢酶(LDH)释放量,丙二醛(MDA)含量,超氧化物歧化酶(SOD)、过氧化氢酶(CAT)及谷胱甘肽过氧化物酶(GSH-Px)活性、B淋巴细胞瘤(Bcl)-2及Bcl-2相关X蛋白(Bax)蛋白表达、细胞外调节蛋白激酶(ERK)1/2磷酸化水平。结果与H_2O_2组比较,右美托咪定低、中、高浓度组细胞活力显著提高,细胞早期及晚期凋亡率显著降低,LDH释放量显著减少,MDA含量显著降低,SOD、CAT及GSH-Px活性显著升高,Caspase3、9活性显著降低,Bcl-2及p-ERK1/2表达量显著上调,Bax表达量显著下调(均P0.01)。结论右美托咪定能通过抗氧化及抗细胞凋亡进而抑制H_2O_2引起的PC12细胞损伤。
[Abstract]:Objective to investigate the protective effect of dexmetomidine on PC12 cell damage induced by S / H _ 2O _ 2.Methods PC12 cells were randomly divided into two groups: normal control group (H _ 2O _ 2) group (n = 200 渭 mol/L / h _ 2O _ 2) and dexmetomidine group (n = 2). #number0# 渭 mol/L dexmetomidine 200 渭 mol/L H _ 2O _ 2 in high concentration group, cultured for 48 h, cell viability, apoptosis, caspase9 activity, lactate dehydrogenase (LDH) release and malondialdehyde (MDA) content were measured. Superoxide dismutase (SOD), catalase (catalase) and glutathione peroxidase (glutathione peroxidase) (GSH-Px) activity and the expression of Bcl-2and Bcl-2 related X protein (Bax-2) protein, and the phosphorylation level of extracellular regulated protein kinase (ERK) 1 / 2 were compared with those in group H _ 2O _ 2. In the low, middle and high concentration groups of dexmetomidine, the cell viability was significantly increased. Apoptosis rate in early and late stage of cells significantly decreased LDH release, decreased MDA content, significantly decreased activity of cat and GSH-Px, significantly increased the activity of Caspase3P9, significantly decreased the expression of Bcl-2 and p-ERK 1 / 2, significantly up-regulated the expression of Bax (all P 0.01). Tomidine can inhibit PC12 cell damage induced by H _ 2O _ 2 by anti-oxidation and anti-apoptosis.
【作者单位】：福建省肿瘤医院福建医科大学附属肿瘤医院麻醉科;
【基金】：国家自然科学基金青年科学基金项目(No.81400921) 人社部2016年度留学人员科技活动项目择优资助项目(2016年)
【分类号】：R614

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