非化脓性关节炎患者炎性介质在血清及关节滑膜中的表达与CRP的相关性研究

发布时间：2018-03-25 10:24

  本文选题：CRP　切入点：骨性关节炎　出处：《新乡医学院》2015年硕士论文

【摘要】：背景非化脓性关节炎(Non-suppurative arthritis)是一种常见的以关节组织慢性炎症性变为主要表现的全身性疾病,本病侵犯多个关节。非化脓性关节炎包括血友病性关节炎、痛风性关节炎、大骨节病、类风湿性关节炎、强直性脊柱炎及骨性关节炎等。而骨性关节炎(osteoarthritis,OA)和类风湿性关节炎(Rheumatoid arthritis,RA)在临床上较为多见。我国作为一个人口大国,伴随着老龄化社会的到来,60岁以上人口更是超过了1亿,而未来将会出现越来越多的OA、RA患者。目的研究非化脓性关节炎患者(以OA、RA患者为主要研究对象)血清及膝关节滑膜中IL-1、IL-6和TNF-α等炎症因子指标与C反应蛋白(C reactive protein,CRP)轻度增高(CRP介于10-30mg/L之间)之间的相关性。方法选取2013年1月到2014年1月在新乡医学院第一附属医院入院,并通过全面的体格检查和影像学检查确诊为骨性关节炎和类风湿性关节炎的患者。选取CRP轻度增高的骨性关节炎和类风湿性关节炎患者10例(CRP介于10-30mg/L之间)为实验组。另选取CRP处于正常值(CRP≤10mg/L)的骨性关节炎和类风湿性关节炎患者10例,为对照组。采用ELISA法检测两组患者血清中IL-1、IL-6及TNF-α的水平。采用免疫组化等方法检测两组患者关节滑膜中IL-1、IL-6及TNF-α的水平。应用HE石蜡染色法镜下分析两组患者膝关节滑膜表达情况,从三种实验中得出结果,并结合CRP值对实验组和对照组进行对比及分析。结果1.通过ELISA法检测,实验组患者血清中IL-1、IL-6及TNF-α的水平表达明显高于对照组。2.通过免疫组化方法检测,患者膝关节滑膜中IL-1、IL-6及TNF-α的水平表达明显高于对照组。3.HE染色关节滑膜组织镜下对比,实验组镜下阳性表达高于对照组,与CRP的轻度增高呈正相关性。结论1.OA、RA患者血清及膝关节滑膜中IL-1、IL-6、TNF-α等炎症因子水平的增高与术前无感染灶的情况下CRP的轻度增高相关。2.为临床选择合适的手术时机,降低关节置换手术的感染率提供一定的理论依据。
[Abstract]:Background Non-suppurative arthritis (non-suppurative arthritis) is a common systemic disease characterized by chronic inflammation of the articular tissue, which invades multiple joints. Non-suppurative arthritis includes hemophilia arthritis and gouty arthritis. Kaschin-Beck disease, rheumatoid arthritis, ankylosing spondylitis and osteoarthritis. With the advent of an aging society, more than 100 million people are over 60 years old. In the future, there will be more and more patients with OAA. Objective to study the relationship between serum and synovial cytokines such as IL-1-, IL-6, TNF- 伪 and C-reactive protein C reactive protein (CRP) in patients with non-suppurative arthritis (OA-RA). Methods from January 2013 to January 2014, they were admitted to the first affiliated Hospital of Xinxiang Medical College. The patients with osteoarthritis and rheumatoid arthritis were diagnosed by comprehensive physical examination and imaging examination. Ten patients with mild increase of CRP were selected to be between 10-30mg/L and Osteoarthritis and Rheumatoid Arthritis (RA). In addition, 10 patients with osteoarthritis and rheumatoid arthritis (CRP 鈮，

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