兔膝骨性关节炎治疗中透明质酸钠与曲安奈德对滑膜和软骨的影响

发布时间：2018-03-26 10:26

本文选题：骨性关节炎　切入点：关节软骨　出处：《海南医学》2016年21期

【摘要】：目的评估透明质酸钠与曲安奈德对兔膝骨性关节炎中关节滑膜及软骨的影响。方法 24只新西兰兔制造骨关节炎模型后按数表法随机分为对照组、透明质酸钠(SH)组、曲安奈德(TA)组,每组各8只,分别进行关节腔注射生理盐水0.4 m L、SH 0.4 m L、TA 0.4 m L,每周一次,每组6次。6周后观察每组兔膝关节滑膜组织病理切片、血管滑膜SP法染色、软骨AB-PAS染色及MMP-1免疫组化。结果滑膜病理切片显示,SH组滑膜增生最少,TA组滑膜增生较少,对照组滑膜增生明显;滑膜SP法染色显示SH组、TA组VEGF阳性细胞百分数较对照组减少[(42.50±5.29)%、(50.80±5.24)%vs(53.38±4.75)%,P0.05],SH组、TA组平均光密度值较对照组减少[(0.32±0.02)、(0.43±0.04)vs(0.48±0.05),P0.05],TA组较SH组和对照组内软骨细胞减少,软骨基质成分减少,SH组、TA组较对照组软骨表层破坏减少。SH组、TA组软骨MMP-1细胞百分数评分优于对照组[(61.25±4.06)%、(76.27±4.68)%vs(87.13±3.64)%,P0.05];SH组、TA组软骨Mankins评分优于对照组[(6.88±1.25)分、(7.27±0.68)分vs(8.13±0.83)分,P0.05]。结论透明质酸钠和曲安奈德均能抑制滑膜炎增生,减少兔膝关节腔内的炎性细胞因子,减少了软骨基质的破坏,且透明质酸钠要优于曲安奈德。
[Abstract]:Objective to evaluate the effects of sodium hyaluronate and triamcinolone acetonide on synovitis and cartilage in rabbit knee osteoarthritis. In each group, 8 rats in each group were injected with normal saline 0.4 mL of SH 0.4 mL of TA once a week. After 6 weeks in each group, pathological sections of synovial tissue were observed in each group, and SP staining of vascular synovium was performed in each group. Results AB-PAS staining and MMP-1 immunohistochemistry showed that synovial hyperplasia was less in SH group than in TA group, while that in control group was obvious. SP staining of synovium showed that the percentage of VEGF positive cells in SH group was lower than that in control group [42.50 卤5.29 + 50.80 卤5.24)%vs(53.38 卤4.75] the mean optical density of TA group in SH group was lower than that in control group [0.32 卤0.02 0.04)vs(0.48 卤0.05] the percentage of VEGF positive cells in TA group was lower than that in SH group and control group. The percentage of chondrocytes in TA group was better than that in control group [61.25 卤4.06%] the Mankins score in TA group was better than that in control group [7.27 卤0.68) vs(8.13 卤0.83]. Conclusion Hyaluronic acid is superior to hyaluronic acid in cartilage Mankins score (P 0.05). Sodium and triamcinolone acetonide both inhibited synovitis proliferation. The reduction of inflammatory cytokines in rabbit knee joint reduced the destruction of cartilage matrix, and sodium hyaluronate was superior to triamcinolone acetonide.
【作者单位】：北京大学第四临床医学院北京积水潭医院运动损伤科;北京大学深圳医院运动医学与康复科;
【基金】：广东省深圳市医疗卫生科研项目(编号:201302057)
【分类号】：R684.3

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