EPO治疗对大鼠脊髓损伤后脊髓运动功能恢复的实验研究

发布时间：2018-03-28 05:40

本文选题：脊髓损伤　切入点：促红细胞生成素　出处：《安徽医科大学学报》2016年12期

【摘要】：目的探讨人重组促红细胞生成素(rh-EPO)在大鼠脊髓损伤(SCI)后运动功能恢复中的作用。方法选取30只200~230 g成年雄性SD大鼠,随机分为假手术(Sham)组、脊髓损伤(SCI)组和EPO组,每组10只。Sham组仅行椎板切除术,不伤及脊髓。SCI组和EPO组行椎板切除及脊髓打击术。术后EPO组腹腔注射rh-EPO,SCI组和Sham组采用相同剂量的生理盐水。应用BBB评分评估大鼠术后第1、3、7、14天的后肢运动功能恢复情况。HE染色观察术后第14天脊髓形态学变化;免疫荧光测定新生神经元的增殖情况。结果术后第1、3天SCI组和EPO组之间BBB评分差异无统计学意义;术后第7、14天EPO组BBB评分明显高于SCI组(P0.05,P0.01)。SCI组可见大量胶质瘢痕、空洞形成和炎性细胞浸润;EPO组上述改变较SCI组减轻。Sham组、SCI组和EPO组新增殖神经元数目逐渐增多,各组间比较差异有统计学意义(P0.01)。结论大剂量rhEPO的治疗可减轻脊髓内炎症反应、刺激脊髓新生神经元的增殖和促进大鼠SCI后后肢运动功能的恢复。
[Abstract]:Objective to investigate the role of recombinant human erythropoietin rh-EPO in the recovery of motor function after spinal cord injury (SCI) in rats. Methods 30 adult male SD rats with spinal cord injury were randomly divided into three groups: sham group, sci group and EPO group. 10 rats in each group were treated with laminectomy only. No spinal cord injury. Sci group and EPO group were treated with laminectomy and spinal cord strike. After operation, rh-EPO-sci group and Sham group were injected intraperitoneally with the same dose of normal saline. BBB score was used to evaluate the recovery of motor function of the hind limbs of rats on the 1st day, 3rd day, 7th day after operation. The morphological changes of spinal cord were observed on the 14th day after operation by HE staining. Results there was no significant difference in BBB score between SCI group and EPO group on the 1st day after operation, the BBB score in EPO group was significantly higher than that in SCI group on 714 days after operation, and a large number of glial scars were found in sci group. Compared with SCI group, the number of new proliferative neurons in sci group and EPO group increased gradually, and the difference was statistically significant (P 0.01). Conclusion the treatment of high dose rhEPO can reduce the inflammatory response in spinal cord. To stimulate the proliferation of spinal neonate neurons and promote the recovery of hind limb motor function after SCI in rats.
【作者单位】：安徽医科大学第一附属医院骨科;安徽医科大学第四附属医院骨科;
【基金】：国家自然科学基金(编号:81171173) 安徽省自然科学基金(编号:11040606Q25)
【分类号】：R651.2

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