兔颈脊髓半侧挫伤模型的建立及其MRI和组织学表现的研究

发布时间：2018-03-29 22:10

本文选题：颈脊髓损伤　切入点：单侧挫伤　出处：《中国临床解剖学杂志》2017年01期

【摘要】：目的建立兔颈脊髓半侧挫伤模型,观察不同程度损伤24小时后其MRI及组织学表现。方法 22只成年雄性新西兰兔,随机分为中度损伤组(n=9)、重度损伤组(n=9)和假手术组(n=4)。直径为3.0 mm的打击头由电磁伺服材料试验机驱动,对准C_5脊髓左侧行高速挫伤(500 mm/s)。根据打击头的位移距离分为位移2.0 mm组(中度损伤组)和位移2.8 mm组(重度损伤组)。假手术组仅暴露C_5脊髓,不进行挫伤。损伤后24小时每组随机取两例行MRI影像学检查,所有动物均取材进行组织学观察,测量横断面脊髓出血面积。结果中度损伤组打击力和位移分别为(2.47±0.39)N和(1.99±0.02)mm,重度损伤组打击力和位移分别为(5.16±0.82)N和(2.76±0.02)mm,中度损伤组的打击力明显小于重度损伤组(P0.05)。MRI结果显示,中度及重度损伤组均可见C_5左侧脊髓信号改变。HE染色显示脊髓左侧有明显的出血及脊髓组织结构破坏,中度损伤组损伤中心横截面出血面积(0.012±0.006)mm~2明显小于重度损伤组(0.039±0.006)mm~2(P0.05)。结论本文建立的兔颈脊髓半侧挫伤模型能够控制挫伤位移,实现对脊髓的高速打击。不同程度的颈脊髓半侧挫伤在打击力、MRI影像学及组织学上均有不同。
[Abstract]:Objective to establish a rabbit model of cervical spinal cord contusion, and to observe the MRI and histological features of rabbits after 24 hours of different degree of injury. Methods 22 adult male New Zealand rabbits were studied. They were randomly divided into moderate injury group, severe injury group and sham-operation group. The hit head with a diameter of 3.0 mm was driven by electromagnetic servo material testing machine. The left side of C5 spinal cord was subjected to high speed contusion (500 mm / s). According to the displacement distance of the head, it was divided into two groups: displacement 2.0 mm group (moderate injury group) and displacement 2.8 mm group (severe injury group). The sham operation group only exposed C5 spinal cord. No contusion was performed. Two MRI imaging examinations were performed at random in each group 24 hours after injury, and all animals were taken for histological observation. Results the impact force and displacement were 2.47 卤0.39 and 1.99 卤0.02 mm in the moderate injury group, and 5.16 卤0.82N and 2.76 卤0.02 mm in the severe injury group, respectively. The impact force in the moderate injury group was significantly lower than that in the severe injury group (P 0.05). In the moderate and severe injury groups, the signal changes of the left spinal cord were observed. He staining showed that the left side of the spinal cord had obvious hemorrhage and the structure of the spinal cord was damaged. The cross section hemorrhage area of the injury center in the moderate injury group was 0.012 卤0.006)mm~2 less than that in the severe injury group. Conclusion the contusion model of cervical spinal cord can control the contusion displacement. Different degree of contusion of cervical spinal cord had different MRI imaging and histology.
【作者单位】：南方医科大学南方医院脊柱骨科;
【基金】：国家自然科学基金(81472084)
【分类号】：R651.2;R-332

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