公兔骶髓缺血损伤动物模型的建立及评估

发布时间：2018-03-31 12:04

  本文选题：球海绵体肌反射　切入点：骶髓缺血损伤　出处：《中国神经精神疾病杂志》2017年07期

【摘要】：目的建立公兔不同水平骶髓缺血损伤动物模型并应用球海绵体肌反射(bulbocavernosus sphincter reflex,BCR)对模型进行评估。方法 36只新西兰大白兔公兔随机区组法分为实验组(n=30)和对照组(n=6),实验组按照尾头方向结扎左肾动脉和动脉分叉间腰动脉的水平分为1根、2根、3根、4根和5根组共5亚组(n=6),分别结扎1根、2根、3根、4根和5根腰动脉,记录血管结扎后2 h及2 d的BCR波,分别在麻醉清醒和血管结扎后2 d进行运动功能评分,2 d后取骶髓HE切片观察。对照组(n=6)用于排除麻醉、手术对BCR的影响。结果对照组麻醉和手术后不同时间的波幅应用单组重复测量数据的方差分析,差异均无统计学意义(F=1.234和F=1.492,均P0.05);对照组麻醉和手术后不同时间的潜伏期应用单组重复测量数据的方差分析,差异均无统计学意义(F=0.911和F=3.80,均P0.05)。实验组术前、结扎后2 h和结扎后2 d三个时间点BCR波幅与基线波幅的百分比值,经单因素重复测量数据的方差分析差异有统计学意义(F=1029.943,P0.05);实验组术前、结扎后2h和结扎后2 d三个时间点BCR潜伏期,经单因素重复测量数据的方差分析,结果差异有统计学意义(F=22.711,P0.05)。1根组、2根组手术前后和3根组术后2 d运动功能评分无变化;3根组麻醉清醒后和4根、5根组手术后不同时间运动功能评分与对照组和手术前经Wilcoxon秩和检验均P0.05,差异均有统计学意义。结论通过尾头方向分别结扎左肾动脉和动脉分叉间不同水平的腰动脉可以建立不同程度的骶髓缺血损伤动物模型。
[Abstract]:Objective to establish an animal model of sacral spinal cord ischemia injury in male rabbits at different levels and evaluate the model by bulbocavernosus sphincter reflexus.Methods Thirty-six New Zealand white rabbits were randomly divided into two groups: the experimental group (30) and the control group (6 cases). The experimental group was divided into two groups according to the level of ligating left renal artery and the artery bifurcation lumbar artery in the direction of tail head: 1, 2, 3, 3, 4 and 5, respectively.A total of 5 subgroups were ligated, including 1, 2, 3, 4 and 5 lumbar arteries, respectively.The BCR waves were recorded at 2 hours and 2 days after ligation. The motor function scores were scored 2 days after anesthesia and 2 days after vascular ligation. The HE sections of the sacral spinal cord were taken for observation.Control group (n = 6) was used to exclude the effect of anesthesia and operation on BCR.Results in the control group, the amplitudes at different times after anesthesia and operation were analyzed by ANOVA.There was no significant difference (P < 0.05) between the two groups (P < 0.01), but there was no significant difference between the two groups (P < 0.05). The latency of anesthesia and operation in the control group was analyzed by ANOVA of repeated measurements at different time after anaesthesia and operation. There was no significant difference between the two groups (P < 0.01), but there was no significant difference between the two groups (P < 0.05).The percentage of BCR amplitude and baseline amplitude at three time points before ligation, 2 hours after ligation and 2 days after ligation were significantly different from baseline amplitude by ANOVA of single factor repeated measurement data.The latency of BCR at 2 hours after ligation and 2 days after ligation was analyzed by ANOVA.Results there was significant difference in motor function score between two groups and three groups before and after operation and 2 days after operation. Motor function scores of 3 groups after anesthesia and 4 groups with 5 roots after operation were similar to those of control group and hand group at different time after operation.The Wilcoxon rank sum test showed significant difference before operation (P 0.05).Conclusion the animal model of sacral spinal cord ischemia injury can be established by ligating the left renal artery and the lumbar artery at different levels in the direction of the tail head.
【作者单位】： 青岛大学附属医院病理科;山东省临沂市人民医院神经外科;
【基金】：山东医药卫生科技发展计划面上项目(编号:2015WS0375)
【分类号】：R-332;R651.2
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本文编号：1690652