槲皮素对兔骨关节炎模型关节液中IL-1β、TNFα水平的影响

发布时间：2018-04-01 06:36

本文选题：骨关节炎　切入点：槲皮素　出处：《桂林医学院》2017年硕士论文

【摘要】：目的:观察目前槲皮素对骨关节炎动物模型关节液中细胞因子IL-1β、TNF-α水平的影响。方法:将20只新西兰大白兔按照hulth法建立兔膝关节骨关节炎模型,随机等分成4组,每组各5只。(1)槲皮素组(2)塞来昔布组(3)生理盐水组(4)模型组。建模4周后各治疗组分别给予药物灌胃治疗,槲皮素组建模开始灌槲皮素10.31mg/(kg·d),塞来昔布组10.31mg/(kg·d),生理盐水组10.31ml/(kg·d),模型组不给予药物灌胃治疗,连续灌胃4周。4周后给予空气栓塞处死,抽取膝关节液用ELISA法检测关节液中IL-1β、TNF-α的含量,并取股骨内髁关节软骨标本送普通病理检查及做膝关节大体评分。结果:(1)模型组股骨内髁处可见软骨表面软骨剥脱,深达骨质,关节内有骨赘形成。HE染色可见骨组织及软骨细胞较少。(2)槲皮素、塞来昔布治疗组膝关节大体评分显著低于模型组及生理盐水组(P0.05),槲皮素、塞来昔布组间大体评分无差异(P0.05)。(3)槲皮素组、塞来昔布组膝关节液中IL-1β、TNF-α水平显著低于模型组及生理盐水组(P0.05)。结论:槲皮素、塞来昔布均能够抑制OA模型关节软骨破坏及关节滑液中IL-1β、TNF-α的水平。
[Abstract]:Objective: to observe the effect of quercetin on the level of cytokine IL-1 尾 TNF- 伪 in the articular fluid of osteoarthritis animal model. Methods: twenty New Zealand white rabbits were randomly divided into 4 groups according to the hulth method to establish rabbit knee osteoarthritis model. Each group (n = 5) quercetin group (n = 2) celecoxib group (n = 3) normal saline group (n = 4). Quercetin group was treated with quercetin 10.31mg/(kg dronium, celecoxib group with 10.31mg/(kg dronium and normal saline group with 10.31ml/(kg dronium. The model group was given no drug infusion and was killed by air embolization for 4 weeks. The content of IL-1 尾 -TNF- 伪 in articular fluid was determined by ELISA method in knee joint fluid. The specimens of femoral medial condylar articular cartilage were taken for general pathological examination and knee joint gross score. Results cartilage surface cartilage peeling was seen in the femoral medial condyle in the model group. Osteophyte formation. He staining showed that there were fewer bone tissues and chondrocytes. Quercetin. The gross score of knee joint in celecoxib treated group was significantly lower than that in model group and saline group (P 0.05), quercetin, and quercetin. There was no difference in gross score between celecoxib group (P 0.05) Quercetin group and celecoxib group had significantly lower IL-1 尾 -TNF- 伪 level in knee joint fluid than model group and normal saline group (P 0.05). Conclusion: quercetin, celecoxib group, celecoxib group, celecoxib group, celecoxib group and normal saline group, Celecoxib could inhibit the destruction of articular cartilage and the level of IL-1 尾 -TNF- 伪 in synovial fluid of OA model.
【学位授予单位】：桂林医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R684.3

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