Mfn-2表达和PI3K-Akt通路在七氟醚后处理减轻大鼠心肌缺血再灌注损伤中的作用

发布时间：2018-04-01 17:22

本文选题：七氟醚后处理　切入点：心肌缺血再灌注损伤　出处：《山西医科大学》2017年硕士论文

【摘要】：目的:探讨Mfn-2表达和PI3K-Akt通路在七氟醚后处理减轻大鼠心肌缺血再灌注损伤中的作用。方法:将30只雄性SD大鼠随机分为五组(n=6):假手术(SH)组、心肌缺血再灌注损伤(MIRI)组、七氟醚后处理(SP)组、七氟醚+PI3K通道抑制剂Wortmannin(SP+W)组与Wortmannin(W)组。麻醉固定大鼠并暴露心脏,除外SH组,其余四组均结扎冠脉左前降支30min,复灌120min;SP组与SP+W组在复灌前10min吸入2.3%七氟醚15min;SP+W组在复灌前15min前经股静脉注射稀释的渥曼青霉素(Wortmannin,15ug/kg);W组则只在复灌前15min静注等量该抑制剂。全程记录心电图。结束后检测指标:ELISA法测cTn-I;Western blot法检测P-Akt(激活的Akt)、T-Akt(总Akt)并计算二者的比值,测Mfn-2的表达;并在光镜下观察心肌细胞形态学的变化。结果:各组T-Akt表达差异无统计学意义(P0.05)。与SH组比较,余四组cTn-I含量表达上调,差异有统计学意义(P0.05);与SP组比较,MIRI组、SP+W组以及W组的cTn-I表达上调,差异有统计学意义(P0.05);MIRI组、SP+W组以及W组间的cTn-I含量表达差异无统计学意义(P0.05)。与SH组比较,余四组P-Akt表达上调,差异有统计学意义(P0.05);与SP组比较,MIRI组、SP+W组以及W组的P-Akt表达下调,差异有统计学意义(P0.05);MIRI组、SP+W组以及W组间的P-Akt表达差异无统计学意义(P0.05)。与SH组比较,余四组Mfn-2表达上调,差异有统计学意义(P0.05);与SP组比较,MIRI组与W组的Mfn-2表达上调,差异有统计学意义(P0.05);SP组与SP+W组的Mfn-2表达差异无统计学意义(P0.05),MIRI组与W组的Mfn-2表达差异无统计学意义(P0.05)。同时,光镜下细胞形态差异明显,SH组无受损迹象,与SP组相比较,MIRI组、SP+W组以及W组受损更明显。结论:1.七氟醚后处理减轻大鼠心肌缺血再灌注损伤可能与下调Mfn-2表达、激活PI3K-Akt通路有关。2.本实验七氟醚减轻MIRI的机制中,下调Mfn-2表达不在激活PI3K-Akt通路的下游。
[Abstract]:Aim: to investigate the role of Mfn-2 expression and PI3K-Akt pathway in reducing myocardial ischemia-reperfusion injury after sevoflurane treatment in rats.Methods: thirty male Sprague-Dawley rats were randomly divided into five groups: sham operation group, myocardial ischemia reperfusion injury group, sevoflurane post-treatment group, sevoflurane PI3K channel inhibitor Wortmannin(SP W group and Wortmanningnan group.Rats were anesthetized and exposed to heart, except SH group,The electrocardiogram was recorded.At the end of the study, the expression of Mfn-2 and the ratio of P-Akt (activated Akttium-Akt) were measured by the Western blot method, and the morphological changes of cardiomyocytes were observed under light microscope.Results: there was no significant difference in T-Akt expression in all groups (P 0.05).Compared with SH group, the expression of cTn-I was up-regulated in the other four groups, the difference was statistically significant (P 0.05), and the expression of cTn-I was up-regulated in SP-W and W groups compared with SP group.There was no significant difference in the expression of cTn-I between SP W group and W group.Compared with SH group, the expression of P-Akt in the other four groups was up-regulated, the difference was statistically significant (P 0.05), and the expression of P-Akt was down-regulated in SP W group and W group compared with SP group. There was no significant difference in P-Akt expression between SP W group and W group compared with SP group.Compared with SH group, the expression of Mfn-2 in the other four groups was up-regulated, the difference was statistically significant (P 0.05), and the expression of Mfn-2 was up-regulated in the other four groups compared with SP group and W group.There was no significant difference in Mfn-2 expression between SP group and SP W group. There was no significant difference in Mfn-2 expression between P0.05 and W group.At the same time, there was no significant difference in cell morphology under light microscope in SH group. Compared with SP group, the damage of MIRI group was more obvious than that of SP-W group and W group.Conclusion 1.The protective effect of sevoflurane on myocardial ischemia-reperfusion injury in rats may be related to down-regulation of Mfn-2 expression and activation of PI3K-Akt pathway.In this study, the down-regulation of Mfn-2 expression was not downstream of the activation of PI3K-Akt pathway in the mechanism of sevoflurane alleviating MIRI.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R614

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