术后疼痛对大鼠认知功能障碍的影响及其机制

发布时间：2018-04-01 20:30

本文选题：认知功能障碍　切入点：疼痛　出处：《山东医药》2017年36期

【摘要】：目的探讨术后疼痛对大鼠认知功能障碍的影响及其机制。方法选择SD大鼠80只,采用随机数字表法分为模型组、对照组,每组40只。模型组右后足按Brennan法制作趾部切口疼痛模型,对照组仅麻醉,趾部不制作疼痛模型。模型组术后1(t_1)、3(t_2)、7(t_3)、14天(t_4),对照组同时间,各随机取10只,分别检测右后足机械缩足阈值(MWT)、热缩足潜伏期(TWL),通过Morris水迷宫实验检测穿越平台次数、平台停留时间。检测完上述指标,断头处死,取海马区脑组织,采用Western blotting法检测突触后膜致密蛋白95(PSD95)、N-甲基-D-天冬氨酸受体2B亚型(NR2B)表达。结果模型组t1~t4MWT、TWL均低于对照组同期,穿越平台次数、平台停留时间均低于对照组同期,海马区PSD95、NR2B相对表达量均低于对照组同期(P均0.05)。结论术后疼痛可导致大鼠认知功能障碍发生,其机制可能与疼痛刺激使海马区PSD95、NR2B表达降低有关。
[Abstract]:Objective to investigate the effect and mechanism of postoperative pain on cognitive dysfunction in rats. Methods 80 SD rats were randomly divided into two groups: model group and control group. There were 40 rats in each group. The right hind foot of the model group was made into the pain model of the toe incision according to the Brennan method, while the control group was anesthetized only, but no pain model was made in the toe. In the model group, 10 rats were randomly selected at the same time as the control group. The threshold of mechanical foot contraction in the right hind foot and the latent period of thermal contraction were detected respectively. The times of passing through the platform and the time of staying on the platform were detected by Morris water maze experiment. The above indexes were detected, the head was cut off, and the brain tissue of the hippocampus was taken. The expression of N- methyl-Daspartic acid receptor 2B subtype NR2B) was detected by Western blotting. Results the t1t4MWT-TWL in the model group was lower than that in the control group, the times of crossing the platform and the residence time of the platform were lower than those in the control group. The relative expression of NR2B in hippocampal area was lower than that in control group (P < 0.05). Conclusion postoperative pain may lead to cognitive dysfunction in rats, and the mechanism may be related to the decrease of PSD95 NR2B expression induced by pain stimulation.
【作者单位】：儋州市人民医院;
【分类号】：R614

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