大鼠脊髓压迫性损伤解压后pEGFR的表达变化

发布时间：2018-04-02 04:25

  本文选题：脊髓压迫性损伤　切入点：髓鞘　出处：《中国临床解剖学杂志》2017年02期

【摘要】：目的探讨大鼠脊髓压迫性损伤(compressed spinal cord injury,CSCI)解压后表皮生长因子受体(phosphorylated epidermal growth factor receptor,pEGFR)、pAkt1的表达变化及其与神经功能、有髓神经纤维数量变化的相关性,为CSCI解压后治疗策略的制定和药物的研发提供实验基础。方法采用自行设计的方法制作SD大鼠CSCI模型,造模成功后解压。运用BBB(Basso Beattie Bresnahan)评分观察动物解压后神经功能的恢复情况;通过Luxol fast blue(LFB)染色检测解压后1、7、14、21 d有髓神经纤维数量变化;运用免疫荧光双标(Double-labeling immunoflurescence)、免疫印迹(western blotting,WB)检测pEGFR、pAkt1的表达变化。结果 CSCI解压后,BBB评分和有髓神经纤维数量随时间延长而逐渐增加;与此同时,pEGFR、pAkt1表达亦上调且与BBB评分、有髓神经纤维数量增加趋势一致。结论CSCI解压后,神经功能有一定改善、有髓神经纤维数量有一定增加,这些变化与pEGFR表达上调有关,提示EGFR的活化参与了CSCI解压后的内源性修复。
[Abstract]:Objective to investigate the changes of EGF receptor phosphorylated epidermal growth factor receptor pEGFRR pAkt1 after decompression of compressed spinal cord injury-induced spinal cord injury in rats and its correlation with the changes of nerve function and the quantity of myelinated nerve fibers. Methods SD rat CSCI model was made by using self-designed method, and the model was successfully established. The recovery of nerve function after decompression was observed by BBB(Basso Beattie Bresnahan score. The number of myelinated nerve fibers was detected by Luxol fast blue LFB staining on the 1st day, 1421 d after decompression. The expression of pEGFRN pAkt1 was detected by double labeling and Western blotting with immunofluorescence. Results the CSCI score and the number of myelinated nerve fibers increased gradually with time after decompression, and the expression of pEGFRR pAkt1 was also up-regulated and correlated with BBB score. Conclusion the number of myelinated nerve fibers and the number of myelinated nerve fibers increased after CSCI decompression. These changes are related to the upregulation of pEGFR expression. The results suggest that the activation of EGFR is involved in the endogenous repair after decompression of CSCI.
【作者单位】： 重庆医科大学神经科学研究中心;重庆医科大学附属第一医院璧山医院神经外科;
【基金】：重庆市研究生科研创新项目(40010200100410) 国家青年科学基金(81403466)
【分类号】：R744
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