右美托咪定对淤胆并肝纤维化大鼠肝脏缺血再灌注损伤的影响

发布时间：2018-04-13 09:45

本文选题：右美托咪定 + 肝脏　；参考：《湖南师范大学》2015年硕士论文

【摘要】：目的:探讨右美托咪定(Dexmedetomidine,Dex)对胆汁淤积并肝纤维化大鼠肝脏缺血再灌注损伤(Ischemia Reperfusion Injury,IRI)的影响。方法:采用胆总管结扎(Bile Duct Ligation,BDL)法建立淤胆并肝纤维化大鼠模型,将32只雄性成模SD大鼠随机分为4组,每组8只:①假手术组(S组):开腹显露肝蒂,经腹腔注射生理盐水(Nromal Saline,NS)5mL/kg,不阻断肝门,60min后关腹,术毕4h后再次开腹,取血液及肝组织标本并处死大鼠。②缺血再灌注组(IRI组):开腹显露肝蒂,经腹腔注射NS 5mL/kg,30min后阻断肝门,造成肝脏缺血,30min后解除阻断并关腹,后续操作同S组。③10μg/kg Dex+缺血再灌注组(D10组):开腹显露肝蒂,经腹腔注射2μg/mL浓度Dex 5mL/kg,30min后阻断肝门,后续操作同IRI组。④100μg/kg Dex+缺血再灌注组(D100组):开腹显露肝蒂,经腹腔注射20μg/mL浓度Dex 5mL/kg,30min后阻断肝门,后续操作同IRI组。检测指标:取血清检测总胆红素(Total Billirubin,TBIL)、直接胆红素(Direct Billirubin,DBIL)、谷草转氨酶(Aspertate Transaminase,AST)、谷丙转氨酶(Alanine Transaminase,ALT)及肿瘤坏死因子-α(Tumor Necrosis Factor-α,TNF-α)浓度,取新鲜肝组织测定超氧化物歧化酶(Superoxide Dismutase,SOD)、丙二醛(Malondialdehyde,MDA)浓度;制作肝组织石蜡切片,行HE染色及Masson染色观察肝组织病理形态学改变。结果:①血清TBIL及DBIL水平:4组TBIL结果间差异及DBIL结果间差异均无统计学意义(P0.05)。②血清AST及ALT浓度:IRI组、D10组及D100组AST均明显高于S组(P0.05),D10组及D100组AST较IRI组明显下降(P0.05),且D100组AST明显低于D10组(P0.05);IRI组ALT明显高于S组(P0.05),D100组ALT明显低于IRI组(P0.05)。③肝组织SOD活性及MDA浓度:IRI组、D10组及D100组SOD均明显低于S组(P0.05),D10组及D100组SOD均明显高于IRI组(P0.05);IRI组、D10组及D100组MDA均明显高于S组(P0.05),D10组及D100组MDA均明显低于IRI组(P0.05)。④血清TNF-α浓度:IRI组、D10组及D100组TNF-α均明显高于S组(P0.05),D10组及D100组TNF-α均明显低于IRI组(P0.05)。⑤肝组织病理改变:4组肝组织病理切片均出现相同程度胆管增生及纤维化改变,IRI组肝窦内可见明显炎症细胞浸润,肝细胞肿胀,甚至出现坏死灶,而D10组及D100组肝组织损伤程度较IRI组轻。结论:右美托咪定可能通过上调SOD活性、下调TNF-α浓度减轻淤胆并肝纤维化大鼠肝脏缺血再灌注损伤。
[Abstract]:Aim: to investigate the effect of Dexmedetomine Dexine on hepatic ischemia reperfusion injury in rats with cholestasis and hepatic fibrosis.Methods: the rat model of cholestasis and hepatic fibrosis was established by bile duct ligation and bile duct ligation. Thirty-two male SD rats were randomly divided into 4 groups.After 30 minutes of liver ischemia, the liver was unblocked and closed. The subsequent operation was the same as group S (.310 渭 g/kg Dex ischemia / reperfusion group): the liver pedicle was exposed by laparotomy, and the hepatic hilus was blocked by intraperitoneal injection of 2 渭 g/mL Dex (5 mL / kg) for 30 minutes.The follow-up operation was similar to that in the IRI group (.4100 渭 g/kg Dex ischemia / reperfusion group): the liver pedicle was exposed by laparotomy, and the hepatic hilus was blocked by intraperitoneal injection of 20 渭 g/mL Dex 5 mL / kg for 30 minutes. The follow-up operation was the same as that in the IRI group.The pathological changes of liver tissue were observed by HE staining and Masson staining.Results there was no significant difference in serum TBIL and DBIL levels between the four groups of TBIL and DBIL results. There was no significant difference in serum AST and ALT concentration. The AST of D10 group and D100 group was significantly higher than that of S group P0.05% D10 group and D100 group AST was significantly lower than that of IRI group.The pathological sections of liver tissues in the 4 groups showed the same degree of bile duct hyperplasia and fibrosis. The infiltration of inflammatory cells in the hepatic sinuses of IRI group was obvious.Liver cells were swollen and even necrotic, but the degree of liver tissue injury in group D 10 and D 100 was less than that in group IRI.Conclusion: dexmetomidine may reduce hepatic ischemia-reperfusion injury in rats with cholestasis and hepatic fibrosis by upregulating the activity of SOD and down-regulating the concentration of TNF- 伪.
【学位授予单位】：湖南师范大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R614

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