BMP诱导活体内肌腱组织成骨化的研究

发布时间：2018-04-17 00:18

本文选题：BMP + 肌腱　；参考：《中国矫形外科杂志》2017年17期

【摘要】：[目的]探讨活体内BMP诱导肌腱组织成骨化的效果。[方法]30只新西兰兔建立活体内肌腱组织诱导成骨模型,一侧为rh BMP-2处理、对侧为空白对照。术后4、8及12周时采用X线片检测肌腱组织钙化情况,术后12周时对肌腱组织进行大体解剖、组织形态及免疫组化检测分析。[结果]术后4、8及12周时X线片检测发现BMP处理侧各时间点上肌腱组织内可见钙化区域,成骨化范围随时间增加逐渐增大(5.51 mm2、9.44 mm2及20.26mm2,P0.05),钙化区域IOD值亦随时间增加而增高(1 178.56、2 135.26及4 726.72,P0.05)。大体解剖可见BMP处理组肌腱组织增粗、质地变硬,H.E及Masson染色检测见肌腱纤维内细胞浸润,局部区域骨样组织形成,并有osteonectin阳性表达细胞分布。此外,肌腱纤维及骨化区域内均可见软骨样细胞形成。[结论]BMP能够诱导活体内肌腱组织发生成骨化,可能与软骨内成骨途径有关。
[Abstract]:[objective] to investigate the effect of BMP on ossification of tendon tissue in vivo.[methods] 30 New Zealand rabbits were used to establish osteogenic model of tendon tissue in vivo, one side was treated with rh BMP-2, the other side was blank control.The calcification of tendon tissue was detected by X-ray film at 8 and 12 weeks after operation, and the gross anatomy, histological morphology and immunohistochemical analysis were made at 12 weeks after operation.[results] X-ray examination at 8 and 12 weeks after BMP showed that calcification areas could be seen in the superior tendons of the side treated with BMP at different time points.The range of ossification gradually increased with time, 5.51 mm ~ 2 ~ 9.44 mm2 and 20.26 mm ~ (2) ~ (2) P _ (0.05), and the IOD value of calcified area increased with time. The values of IOD in calcified area increased by 11178.56 ~ (2) 135.26 and 4 726.72 ~ (2) (P _ (0.05)).Gross anatomy showed that the tendon tissue in BMP group was thicker, the texture of tendon was stiffened, and the infiltration of cells in tendon fiber and the formation of bone like tissue in local area were detected by using H. E and Masson staining. The positive expression of osteonectin was also found in the distribution of osteonectin positive cells.In addition, chondroid cells were found in tendon fibers and ossification areas.[conclusion] BMP can induce ossification of tendon tissue in vivo, which may be related to endochondral osteogenesis.
【作者单位】：解放军第八十八医院全军骨科中心;山东泰安市中心医院儿童骨科;解放军第八十八医院病理科;
【基金】：中国博士后科学基金项目(编号:2013M532177) 全军青年培育项目(编号:14QNP027) 山东省自然科学基金项目(编号:ZR2014HQ010)
【分类号】：R684

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