光交联水凝胶复合克唑替尼—壳聚糖微球治疗骨关节炎的作用及机制研究

发布时间：2018-04-17 07:08

本文选题：骨关节炎 + 克唑替尼　；参考：《浙江大学》2017年硕士论文

【摘要】：目的:本课题旨在研究光交联水凝胶复合克唑替尼-壳聚糖微球减少软骨退变的作用,从而评价其对骨关节炎的治疗效果。方法:首先,我们制作并评价水凝胶和壳聚糖微球的特性。其次,我们评估了克唑替尼对白细胞介素-1β诱导的软骨退变的影响,克唑替尼能够抑制软骨血管生长,并缓解软骨细胞的退变。最后,我们在C57小鼠上利用前交叉韧带切割模型诱导骨关节炎,然后向关节内注射水凝胶复合克唑替尼-壳聚糖微球。分别在4周和8周收集样本,通过番红-固绿染色以及免疫组化染色检测骨关节炎的进展情况,同时对血管内皮生长因子(VEGF)的表达进行评估。结果:本研究的结果首先表明了在退变软骨中会出现VEGF表达的提高;其次,克唑替尼能通过抑制病理软骨的血管生长,从而延缓软骨退变;最后,水凝胶复合克唑替尼-壳聚糖微球能够显著地延缓小鼠骨关节炎的进展。结论:光交联水凝胶复合克唑替尼-壳聚糖微球是一种可行的骨关节炎疗法。
[Abstract]:Aim: to study the effect of photocrosslinking hydrogel combined with cetinib-chitosan microspheres on cartilage degeneration in order to evaluate the effect of photocrosslinking hydrogel on osteoarthritis.Methods: firstly, we prepared and evaluated the properties of hydrogel and chitosan microspheres.Secondly, we evaluated the effect of cetinib on the cartilage degeneration induced by interleukin-1 尾, which inhibited the growth of chondrovascular and alleviated the degeneration of chondrocytes.Finally, we induced osteoarthritis in C57 mice by anterior cruciate ligament cutting (ACL) model, and then intraarticularly injected hydrogel and kazolini-chitosan microspheres into the joints.Samples were collected at week 4 and week 8 respectively. The progress of osteoarthritis was detected by phenyranin-solid green staining and immunohistochemical staining, and the expression of vascular endothelial growth factor (VEGF) was evaluated.Results: the results of this study first showed that there was an increase in VEGF expression in degenerative cartilage; secondly, ketzolinib could delay cartilage degeneration by inhibiting the vascular growth of pathological cartilage; finally,Hydrogel combined with cetinib-chitosan microspheres could significantly delay the development of osteoarthritis in mice.Conclusion: the photocrosslinked hydrogel combined with cetinib-chitosan microspheres is a feasible therapy for osteoarthritis.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R684.3

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