六磷酸肌醇对人骨肉瘤细胞株MG-63和原代培养成骨细胞增殖的影响

发布时间：2018-04-18 21:18

  本文选题：六磷酸肌醇(IP) + 骨肉瘤细胞株　； 参考：《西安交通大学学报(医学版)》2017年04期

【摘要】：目的研究六磷酸肌醇(inositol hexaphosphate,IP6)对人骨肉瘤细胞株MG-63和原代培养成骨细胞增殖的影响,并探求其抗癌效果的最佳作用浓度。方法原代培养人成骨细胞并鉴定,复苏并常规培养人骨肉瘤细胞株MG-63,MTT法检测不同浓度IP6干预培养时两种细胞的增殖情况,确定IP6的最佳抑癌浓度,并进一步用流式细胞仪检测MG-63细胞周期及凋亡情况。结果当IP6浓度≥1mmol/L时开始抑制MG-63细胞增殖,在4mmol/L时达到最大,并呈时间-剂量依赖性;当IP6浓度为0.5、1mmol/L时,对成骨细胞增殖抑制不明显,2mmol/L时轻度抑制,4mmol/L时导致成骨细胞凋亡。结论 IP6能够抑制人骨肉瘤细胞MG-63的增殖并导致其凋亡,其抗癌的最佳作用浓度为2mmol/L。
[Abstract]:Objective to study the effects of inositol hexaphosphate IP6) on the proliferation of human osteosarcoma cell line MG-63 and primary cultured osteoblasts, and to find out the best concentration of its anticancer effect.Methods Primary culture of human osteoblasts and identification, resuscitation and routine culture of human osteosarcoma cell line MG-63 were used to detect the proliferation of human osteosarcoma cells treated with different concentrations of IP6, and to determine the best inhibitory concentration of IP6.Furthermore, MG-63 cell cycle and apoptosis were detected by flow cytometry.Results when the concentration of IP6 鈮，

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