低氧环境下丙泊酚可增加PC12细胞的凋亡

发布时间：2018-04-19 18:40

本文选题：丙泊酚 + 低氧　；参考：《南方医科大学学报》2017年02期

【摘要】：目的探讨低氧环境下丙泊酚对PC12细胞株凋亡的影响及机制。方法将PC12细胞接种于培养板中,采用随机数字表法,将其随机分为6组:低氧对照组(CH组)、空气对照组(CA组)和氧气对照组(CO组),对照组加入脂肪乳浓度10μmol/L。丙泊酚低氧组(PH组)、丙泊酚空气组(PA组)和丙泊酚氧气组(PO组),丙泊酚浓度为10μmol/L。药物处理完毕后分别放入低氧(5%O_2),空气和氧气(35%O_2)环境的细胞培养箱培养,8 h后流式检测细胞凋亡情况,检测细胞内ROS水平和细胞SOD酶活力。结果与CA组比较,PA组细胞凋亡增加,活性氧簇(ROS)升高,超氧化物歧化酶(SOD)活力增强;与CH组比较,PH组细胞凋亡增加,ROS升高,SOD活力增强;与CO组比较,PO组细胞凋亡增加,ROS升高,SOD活力增强;与PA组比较,PH细胞凋亡增加,ROS升高,SOD活力增强;CO组、CA组和CH组上述各指标差异无统计学意义。结论低氧环境下丙泊酚可通过氧化应激损伤导致PC12细胞凋亡增加。
[Abstract]:Objective to investigate the effect and mechanism of propofol on apoptosis of PC12 cell line under hypoxia. Methods PC12 cells were inoculated on the culture plate and randomly divided into 6 groups: hypoxia control group (Ch group), air control group (CA group) and oxygen control group (CO group). The control group was supplemented with 10 渭 mol / L fat milk. The concentration of propofol was 10 渭 mol / L in propofol hypoxia group, propofol air group (PA group) and propofol oxygen group (10 渭 mol / L). After the drug treatment, the cells were cultured in a cell incubator with hypoxia 5 and O _ 2, air and oxygen, respectively. After 8 hours of culture, the apoptosis of cells was detected by flow cytometry, and the level of ROS and the activity of SOD enzyme in the cells were detected. Results compared with CA group, apoptosis increased, Ros increased, SOD activity increased in PA group and SOD activity in PH group. Compared with CO group, the apoptosis increased in PO group and the activity of SOD was increased in Ros group, but there was no significant difference in the above indexes between CA group and Ch group in CO group compared with PA group. Conclusion propofol can induce apoptosis of PC12 cells through oxidative stress injury.
【作者单位】：重庆医科大学附属儿童医院麻醉科;重庆医科大学附属儿童医院儿科学重庆市重点实验室;重庆医科大学附属儿童医院儿童发育重大疾病国家国际科技合作基地;重庆医科大学附属儿童医院儿科发育疾病研究教育部重点实验室;
【基金】：国家自然科学基金(31200853) 重庆市自然科学基金(cstc2012jj A10036) 重庆市卫计委高端人才后备项目(2015HBRC007) 国家临床重点专科建设项目(国卫办医函[2013]544)~~
【分类号】：R614

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