TGF-β1和α-SMA在人损伤半月板中的表达及意义

发布时间：2018-04-23 03:36

  本文选题：半月板 + TGF-β1　； 参考：《南华大学》2015年硕士论文

【摘要】：[目的]通过观察TGF-β1和α-SMA在人的损伤及正常半月板组织的表达情况,并进行相关性分析,探讨半月板损伤时两者的变化情况。为进一步研究半月板损伤愈合的机制提供实验依据。[方法]收集2014年3月至2014年12月52例确诊为半月板损伤的半月板组织以及11例正常半月板组织,3度损伤半月板组织取自关节镜手术中,急性期17例、慢性期16例;2度损伤半月板组织取自全膝关节置换手术中,慢性期19例,以11例正常半月板组织作为正常对照组。通过免疫组织化学染色方法对TGF-β1和α-SMA在各组中的表达情况进行检测,并按损伤时间、损伤程度、年龄进行分组比较,采用阳性细胞百分数进行定量分析,统计的数据采用SPSS19.0软件分析,并运用方差分析各组差异,直线相关分析法分析TGF-β1和α-SMA表达的相关性。[结果]1.TGF-β1在3度损伤急性期组、3度损伤慢性期组阳性细胞百分数均高于正常组(p0.05);3度损伤急性期组阳性细胞百分数高于3度损伤慢性期组(p0.05)。2.α-SMA在3度损伤急性期组、3度损伤慢性期组阳性细胞百分数均高于正常组(p0.05);3度损伤急性期组阳性细胞百分数高于3度损伤慢性期组(p0.05)。3.TGF-β1在慢性期2度损伤组、慢性期3度损伤组阳性细胞百分数均高于正常组(p0.05);慢性期2度损伤组阳性细胞百分数高于慢性期3度损伤组(p0.05)。4.α-SMA在慢性期2度损伤组、慢性期3度损伤组阳性细胞百分数均高于正常组(p0.05);慢性期2度损伤组阳性细胞百分数高于慢性期3度损伤组(p0.05)。5.TGF-β1、α-SMA在损伤半月板中≥45岁组与45岁组的阳性细胞百分数差异均无统计学意义(p0.05)。6.在各实验组及正常对照组中,TGF-β1的表达与α-SMA的表达之间呈明显正相关性,具有统计学意义(r值=0.971,p0.05)。[结论]1.TGF-β1和α-SMA在3度损伤中急性期的表达升高,随着损伤时间的增加而表达降低。2.TGF-β1和α-SMA在慢性期损伤中,损伤程度越大而表达越低。3.在各实验组与正常对照组中,TGF-β1和α-SMA的表达之间存在正相关性。
[Abstract]:[objective] to observe the expression of TGF- 尾 1 and 伪 -SMA in human and normal meniscus tissues, and to investigate the changes of TGF- 尾 1 and 伪 -SMA in meniscus injury. To provide experimental basis for further study of meniscus healing mechanism. [methods] from March 2014 to December 2014, 52 cases of meniscus tissue diagnosed as meniscus injury and 11 cases of normal meniscus tissue were collected from arthroscopic surgery. The meniscus tissue of 16 patients with chronic meniscus injury was taken from the total knee replacement operation. In the chronic stage 19 cases were treated with normal meniscus tissue as the normal control group. The expression of TGF- 尾 1 and 伪 -SMA in each group was detected by immunohistochemical staining. The expression of TGF- 尾 1 and 伪 -SMA in each group were compared according to the time, degree and age of injury, and the percentage of positive cells was used to quantitatively analyze the expression of TGF- 尾 1 and 伪 -SMA in each group. The statistical data were analyzed by SPSS19.0 software. The correlation between TGF- 尾 1 and 伪 -SMA expression was analyzed by linear correlation analysis. [results] 1. The percentage of positive cells of TGF- 尾 1 in the acute phase of the third degree injury group was higher than that in the control group in the chronic phase of the third degree injury. The percentage of positive cells in the acute phase group was higher than that in the chronic phase group of the third degree injury group. 伪 -SMA was found in the acute phase of the third degree injury group. The percentage of positive cells in the chronic stage group was higher than that in the acute stage group in the normal group, and the percentage of the positive cells in the acute stage group was higher than that in the chronic stage group. 3. TGF- 尾 1 was higher in the second degree injury group in the chronic stage. The percentage of positive cells in the third degree chronic injury group was higher than that in the normal group, and the percentage of positive cells in the second degree chronic injury group was higher than that in the third degree chronic injury group, and 伪 -SMA was higher in the second degree chronic injury group. The percentage of positive cells in the third degree chronic injury group was higher than that in the normal group, and the percentage of positive cells in the second degree chronic injury group was higher than that in the chronic third degree injury group. 5. TGF- 尾 1, 伪 -SMA in meniscus injury 鈮，

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