低载荷机械振动对骨折愈合影响最佳周期选择的实验研究

发布时间：2018-04-24 01:13

本文选题：持续与间歇机械振动 + 骨折愈合　；参考：《吉林大学》2017年硕士论文

【摘要】：目的:本实验通过对持续与间歇低载荷机械振动的研究,探讨骨宏观生物力学性能及骨矿物质元素含量对骨折愈合过程的影响,从而寻求一种促进骨折愈合的最佳振动周期。材料与方法:选取63只12月龄健康雌性小尾寒羊并随机分为7组,每组9只,分为基础对照组和实验组,实验组分为持续振动组和间歇N天振动组(N=1、3、5、7、14),实验动物均进行跖骨骨折手术。在术后2周开始振动实验(F=35Hz、a=0.25g、T=15min),振动6周后处死,剔取羊右跖骨制作样本。选取间歇N天振动组(N=1、3、5、14)进行宏观生物力学性能检测;选取基础对照组、持续振动组、间歇N天振动组(N=1、3、5、7、14)进行骨矿物质元素检测。结果:(一)宏观生物力学性能:1、破坏应力:在骨折线及距骨折线15mm处都是间歇1天振动组破坏应力值最大,且各组间没有统计学差异(p0.05);在距骨折线25mm处间歇5天振动组破坏应力值最大,且各组间没有统计学差异(p0.05);2、极限应变:在骨折线处间歇14天振动组极限应变值最小,且各组间没有统计学差异(p0.05);在距骨折线15mm处和距骨折线25mm处都是间歇3天振动组极限应变值最小,且各组间没有统计学差异(p0.05);3、弹性模量:在骨折线处间歇1天振动组弹性模量值最大,且各组间没有统计学差异(p0.05);在距骨折线15mm处间歇3天振动组弹性模量值最大,且各组间没有统计学差异(p0.05);在距骨折线25mm处间歇5天振动组弹性模量值最大,且各组间没有统计学差异(p0.05)。与本课题组所做基础对照组、持续振动组和间歇7天振动组三组的生物力学实验结果对比:从以上三方面,骨折线处持续振动组的数据最大,距骨折线15mm及25mm处间歇7天振动组数据最大。(二)骨矿物质元素检测:1、骨折线处:各元素的含量较对照组都有不同程度的改变,且一般显示出统计学差异(p0.05)。钙(Ca)、磷(P)元素的含量相对于对照组都有所增加,没有统计学差异(p0.05),而在实验组之间有统计学差异(p0.05),且持续振动组的钙(Ca)、磷(P)含量最大。Ca/P的比值也是持续振动组的最大。2、距骨折线15mm处:各元素的含量较对照组都有不同程度的改变,且一般显示出统计学差异(p0.05)。钙(Ca)、磷(P)元素的含量相对于对照组都有所增加,且有统计学差异(p0.05),实验组之间同样显示有统计学差异(p0.05),且间歇7天振动组的钙(Ca)、磷(P)含量最大。Ca/P的比值也是间歇7天振动组的最大。3、距骨折线25mm处:各元素的含量较对照组都有不同程度的改变,且一般显示出统计学差异(p0.05)。钙(Ca)、磷(P)元素的含量相对于对照组都有所增加,且有统计学差异(p0.05),实验组之间同样显示有统计学差异(p0.05),且间歇7天振动组的钙(Ca)、磷(P)含量最大。Ca/P的比值也是间歇7天振动组的最大。结论:持续与间歇低载荷机械振动能够促进骨折愈合,有利于骨的生长,且间歇7天振动组的效果最好,为促进骨折愈合的最佳周期。
[Abstract]:Objective: to explore the effect of bone biomechanical properties and bone mineral element content on fracture healing process through the study of mechanical vibration of continuous and intermittent low load, so as to find an optimal vibration cycle to promote fracture healing. Materials and methods: 63 healthy female small tail Han sheep of 12 months old were randomly divided into 7 groups, 9 in each group, divided into basic control group and experimental group. The experimental group was divided into continuous vibration group and intermittent N day vibration group. The vibration test was carried out at 2 weeks after operation. The specimen was collected from the right metatarsal bone of sheep after 6 weeks of vibration. The macroscopic biomechanical properties were measured in the intermittent N day vibration group, the basic control group, the continuous vibration group and the intermittent N day vibration group, respectively. Results: (1) Macrobiomechanical property: 1, failure stress: the failure stress in the fracture line and the talus fracture line 15mm is the largest in the vibration group with intermittent 1 day interval. There was no statistical difference between each group (p 0.05), the stress value of the vibration group was the largest at the interval of 5 days from the fracture line 25mm, and there was no statistical difference between the groups (p 0.05). The limit strain: the limit strain value of the vibration group was the smallest at the interval of 14 days at the fracture line. There was no statistical difference among the three groups (p0.05), and the limit strain value was the smallest at the 15mm and 25mm of the talus fracture line, and the limit strain value was the lowest in the group with intermittent 3-day vibration. There was no statistical difference among the three groups, and the elastic modulus was the largest in the vibration group with the interval of 1 day at the fracture line, and there was no statistical difference between the groups, and the elastic modulus value was the largest in the vibration group at the interval of 3 days from the fracture line 15mm, and there was no significant difference between the groups in the value of elastic modulus at the interval of 1 day at the fracture line. There was no statistical difference among the three groups, and the elastic modulus of the vibration group was the largest at the interval of 5 days from the fracture line 25mm, and there was no statistical difference between the groups (p 0.05). The results of biomechanical experiments were compared with those of the basic control group, the continuous vibration group and the intermittent 7 day vibration group. The results of biomechanical experiments were as follows: from the above three aspects, the data of the continuous vibration group at the fracture line were the largest. The data of the group with 7 days of intermittent vibration at 15mm and 25mm of talus fracture line were the largest. (2) Bone mineral elements were detected at 1: 1, fracture line: the contents of each element were different from those of the control group, and generally showed statistical difference (p 0.05). Compared with the control group, the contents of Ca, P, P) increased. There was no statistical difference between the two groups, but there was a statistical difference between the two groups. The ratio of Ca / P, P / P in the continuous vibration group was also the largest in the continuous vibration group, and the 15mm of the talus fracture line: the contents of each element were higher than that of the control group. With varying degrees of change, The statistical difference was generally shown (P 0.05). Compared with the control group, the contents of Ca, P, P) increased. There was also a statistical difference between the two groups (p 0.05), and there was also a significant difference between the experimental groups (p 0.05), and the ratio of Ca / P to P / P in the vibratory group with 7 days of intermittent vibration was also the largest. 3 in the vibratory group on the 7th day interval. At the 25mm of the talus fracture line: the contents of each element. There were different degrees of changes in the dose than those in the control group. The statistical difference was generally shown (P 0.05). Compared with the control group, the contents of Ca, P, P) increased. There was also a significant difference between the two groups (P 0.05), and the ratio of Ca / P to P / P was the largest in the group with 7 days of intermittent vibration, and the ratio of Ca / P was also the highest in the group with 7 days of intermittent vibration. Conclusion: continuous and intermittent low load mechanical vibration can promote fracture healing and is beneficial to bone growth. The effect of intermittent 7 days vibration group is the best, which is the best cycle to promote fracture healing.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R683

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