不同周期低载荷机械振动对骨折愈合影响的纳米级实验研究

发布时间：2018-04-26 09:56

本文选题：骨折愈合 + 间歇振动　；参考：《吉林大学》2017年硕士论文

【摘要】：导言:目前,我国人口结构高龄化态势严重。随着年龄增长和机体衰老,骨质发生生理流失,诱发骨质疏松症及骨质疏松性骨折,严重威胁人类健康。为了预防老龄骨质疏松和促进骨折愈合,开发一种简便、安全、有效、非药物性作用的物理治疗方法是非常有必要。本研究将针对不同周期的间歇性低载荷机械振动对骨折愈合影响进行研究,对骨折线附近骨组织横向和纵向纳米级形态结构及其力学性能进行实验研究,以寻求促进骨重建活动和促进骨折愈合的最佳振动周期。方法:实验以56只12月龄雌性小尾寒羊作为实验对象,平均分成8组:基础对照组、自然愈合组、持续振动组、间歇振动1天组、间歇振动3天组、间歇振动5天组、间歇振动7天组、间歇振动14天组。将35Hz、0.25g低载荷机械振动力学刺激垂直作用于骨折模型上,动物实验持续8周,每日1次,每次15min,其中持续振动组每天接受一次振动,间歇振动X天组,振动X天后休息X天。振动后进行骨样本采集,在骨样本骨折线附近区域采集2mm厚扇状的横向实验检测样本和4mm×4mm×2mm的纵向实验检测样本,分别利用原子力显微镜(AFM)和纳米压痕仪对近端外骨痂、近端内骨痂、远端外骨痂和远端皮质骨四个区域骨组织的纳观形貌和力学性能进行检测。结果:(一)在纳观形貌上:1.横向和纵向颗粒尺寸大小,间歇振动X天组与自然愈合组之间、持续振动组与自然愈合组之间、间歇7天组与持续振动组之间呈现出比较明显的差异性(P0.05),并且间歇7天组的颗粒尺寸最小,间歇1天、3天、5天和14天组次之,自然愈合组最大;2.横向和纵向表面粗糙度,间歇振动X天组与自然愈合组之间、持续振动组与自然愈合组之间、间歇7天组与持续振动组之间呈现出比较明显的差异性(P0.05),并且间歇7天组的粗糙度最大,间歇1天、3天、5天和14天组次之,自然愈合组最小。(二)在纳米级力学性能上:1.横向和纵向样本的弹性模量,间歇X天组与持续振动组之间、持续振动组与自然愈合组之间均未呈现出比较明显的差异性(P0.05),并且间歇7天组弹性模量最大;2.横向和纵向样本的硬度,间歇X天组与持续振动组之间、持续振动组与自然愈合组之间均未呈现出比较明显的差异性(P0.05),并且间歇7天组的硬度最大。结论:纳观形貌分析结果表明,不同的间歇振动周期确实可以改善纳观形貌,间歇振动成骨效应优于持续振动,最佳的振动间歇周期为7天。纳米级力学性能分析结果表明,不同的间歇周期振动可以改善纳米级力学性能,间歇周期为7天的弹性模量最大、硬度最大。综上所述,不同的间歇振动周期能够更好地促进骨重建,更有利于促进骨折的愈合,间歇周期为7天效果最佳。
[Abstract]:Introduction: at present, China's population structure aging situation is serious. With the age and aging of the body, physiological loss of bone occurs, which induces osteoporosis and osteoporotic fracture, which is a serious threat to human health. In order to prevent senile osteoporosis and promote fracture healing, it is necessary to develop a simple, safe, effective and nonpharmacological physical therapy. In this study, the effects of intermittent low-load mechanical vibration on fracture healing were studied, and the transverse and longitudinal nanostructures and mechanical properties of bone near the fracture line were studied experimentally. In order to search for the best vibration cycle to promote bone reconstruction and promote fracture healing. Methods: 56 female small tail Han sheep (12 months old) were divided into 8 groups: basic control group, natural healing group, continuous vibration group, intermittent vibration group for 1 day, intermittent vibration group for 3 days and intermittent vibration group for 5 days. Intermittent vibration group for 7 days, intermittent vibration group for 14 days. The mechanical vibration and mechanical stimulation of 35Hz 0.25g low load mechanical vibration was applied vertically to the fracture model. The animal experiment lasted for 8 weeks, once a day for 15 minutes. The continuous vibration group received one vibration every day, the intermittent vibration X day group, and the rest X day after the vibration X day. After vibration, bone samples were collected. 2mm thick sector test samples and 4mm 脳 4mm 脳 2mm longitudinal test samples were collected in the area near the fracture line of bone samples. The proximal external callus was detected by atomic force microscope (AFM) and nano-indentation instrument, respectively. The morphology and mechanical properties of proximal callus, distal external callus and distal cortical bone were examined. Results: (1) on the morphology of Na, 1: 1. Size of transverse and longitudinal particles, between intermittent vibration X day group and natural healing group, between continuous vibration group and natural healing group, There was a significant difference between the intermittent 7 day group and the continuous vibration group, and the particle size of the interval 7 day group was the smallest, the interval 1 day 3 days 5 days and 14 days group was the second, and the natural healing group was the largest 2. Horizontal and longitudinal surface roughness, between the intermittent vibration X day group and the natural healing group, between the continuous vibration group and the natural healing group, There was a significant difference between the interval 7 day group and the continuous vibration group, and the roughness of the interval 7 day group was the biggest, the interval 1 day 3 days 5 days and 14 days group was the second, and the natural healing group was the least. (2) on the mechanical properties of nanoscale: 1. The elastic modulus of transverse and longitudinal samples, between intermittent X day group and continuous vibration group, and between continuous vibration group and natural healing group showed no significant difference (P 0.05), and the maximum elastic modulus of 7 days interval group was 2. The hardness of transverse and longitudinal samples, intermittent X day group and continuous vibration group, continuous vibration group and natural healing group showed no significant difference (P 0.05), and the hardness of interval 7 day group was the highest. Conclusion: the results of nano morphology analysis show that different intermittent vibration cycles can improve the morphology of the samples, and the effect of intermittent vibration osteogenesis is better than that of continuous vibration, and the best intermittent vibration period is 7 days. The results of mechanical properties analysis show that the mechanical properties of nano-scale can be improved by different intermittent periodic vibration. The elastic modulus and hardness of the seven days interval period are the largest. To sum up, different intermittent vibration cycles can better promote bone reconstruction and promote fracture healing. The interval period of 7 days is the best.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R683

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