以纳米羟基磷灰石—丝蛋白为基础的骨修复材料的设计与合成

发布时间：2018-04-29 05:19

本文选题：纳米材料 + 羟基磷灰石　；参考：《北京化工大学》2016年硕士论文

【摘要】：骨组织损伤问题会给患者带来很大困扰,严重的还会危及生命。人工骨修复材料是解决此类问题的重要途径。仿骨材料则是其中一类用于模仿骨组织功能的骨修复替换材料。本论文从骨组织的组成羟基磷灰石和蛋白出发,结合超重力技术,探究了纳米羟基磷灰石与蛋白/纳米羟基磷灰石复合材料的制备过程及其在骨组织替换材料等生物医学领域的应用潜力。本文主要研究内容如下：(1)本文使用不同磷源与钙源制得纳米羟基磷灰石(nHAP),并利用X射线衍射(XRD)、红外光谱(FTIR)、透射电镜(TEM)、热重分析(TGA)等分析手段对产物进行表征,探究不同反应温度,不同原料等实验条件对产物的组成、形貌、尺寸的影响。结果表明升高温度有利于晶体成长,不同钙源对产物影响不大,不同磷源对得到的产物组成影响较大,磷酸氢根会取代产物羟基磷灰石中的磷酸根,磷酸氢二钠为原料的产物中磷酸氢根含量最高。(2)本文利用超重力沉淀法成功合成出nHAP,并通过XRD和TEM确定了晶体结构与形态,利用FTIR和电感耦合等离子体原子发射光谱(ICP-AES)分析了纳米羟基磷灰石的化学性质。表征结果表明合成的纳米羟基磷灰石直径为1.9-14.2 nm,长度为4.0-36.9 nm,并且与天然骨磷灰石类似,均为碳酸磷灰石结构。另外,本文还对纳米羟基磷灰石在制备磷酸钙骨水泥(CPC)和明胶/HAP水凝胶复合材料方面的潜在应用进行了探讨。(3)本文利用超重力共沉淀法制备了明胶蛋白/纳米羟基磷灰石复合材料并采取多种测试手段表征了材料的理化性质。结果证实了蛋白/HAP复合材料为纳米棒状晶型和具有高蛋白吸附率。此外,蛋白/HAP纳米复合材料还被塑造成圆柱状的CPC,测试结果表明平均抗压模量为0.6 GPa,与人体松质骨类似。同时,利用此法也成功制备出丝蛋白/纳米羟基磷灰石复合材料与CPC,表明此法具有一定的普适性。以上研究结果表明,本文提出的超重力技术结合化学沉淀法的路线具有很多优势,是一种能高效简单、可产业化生产仿骨材料的新工艺。
[Abstract]:Bone tissue injury can cause great trouble and life-threatening for patients. Artificial bone repair material is an important way to solve this problem. Bone imitating material is a kind of bone repair replacement material used to imitate the function of bone tissue. In this paper, the composition of bone tissue hydroxyapatite and protein, combined with hypergravity technology, The preparation process of nano-hydroxyapatite / protein / nano-hydroxyapatite composite and its potential application in biomedical fields such as bone tissue replacement materials were investigated. The main contents of this paper are as follows: (1) Nano-hydroxyapatite (HAP) was prepared by using different phosphorus and calcium sources. The products were characterized by X-ray diffraction (XRD), FTIR (FTIR), TEM (Tem), TGA (thermogravimetric analysis) and so on. The effects of different reaction temperature, different raw materials and other experimental conditions on the composition, morphology and size of the product were investigated. The results show that increasing temperature is beneficial to crystal growth, different calcium sources have little effect on the product, and different phosphorus sources have great influence on the composition of the product. Hydrogen phosphate can replace the phosphate radical in the product hydroxyapatite. In this paper, nHAPs were synthesized by the method of hypergravity precipitation, and the crystal structure and morphology were determined by XRD and TEM. The chemical properties of nano-hydroxyapatite were analyzed by FTIR and ICP-AES-based inductively coupled plasma atomic emission spectrometry (ICP-AES). The results show that the synthesized nano-hydroxyapatite has a diameter of 1.9-14.2 nm and a length of 4.0-36.9 nm, which is similar to that of natural bone apatite. In addition, The potential applications of nano-hydroxyapatite in the preparation of calcium phosphate cement (CPC) and gelatin / HAP hydrogel composites were also discussed. The physical and chemical properties of the composites were characterized by various testing methods. The results show that protein / HAP composites are nanorods and have high protein adsorption rate. In addition, the protein / HAP nanocomposites were molded into cylindrical CPC.The results show that the average compressive modulus is 0.6 GPA, which is similar to that of human cancellous bone. At the same time, silk protein / nano-hydroxyapatite composites and CPC were successfully prepared by this method. The above results show that the proposed route of hypergravity combined with chemical precipitation has many advantages and is a new process for producing bone imitating material with high efficiency and simplicity.
【学位授予单位】：北京化工大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R318.08;R68

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