新型大鼠脊髓打击器的制备及建模大鼠的功能与病理评价

发布时间：2018-05-01 19:20

本文选题：脊髓损伤 + 大鼠　；参考：《中国矫形外科杂志》2017年16期

【摘要】：[目的]制备一种简便、实用的急性大鼠脊髓损伤建模装置。[方法]用木板、新泡沫板、吸管、手术缝线及金属插销杆等制作新型大鼠脊髓打击器。取36只成年雌性SD大鼠,体质量为220~250 g,随机等分为假手术组(Sham组)、轻度打击组(SCI1组)和重度打击组(SCI2组),利用自制大鼠脊髓打击器建立大鼠脊髓损伤模型,SCI1组采用10 g×25 mm打击规格进行垂直打击损伤,SCI2组采用10 g×50 mm打击规格进行垂直打击损伤。各组大鼠于术前1 d,术后1、3、7、14、21和28 d进行BBB运动学评分,观察大鼠后肢运动功能情况,术后28 d对各组大鼠受损脊髓组织进行HE染色、神经元核抗原(Neu N)免疫组化检测。[结果]Sham组各观察时间点的BBB评分均为21分,各SCI组术后1 d时BBB评分为0分,之后BBB评分结果随时间延长呈逐步恢复趋势,从3 d开始,SCI1组的BBB评分高于SCI2组(P0.05)。各组大鼠后肢左右侧活动情况无明显差异,实验过程中无大鼠死亡。术后28 d HE染色、Neu N免疫组化结果显示SCI2组脊髓受损程度明显重于SCI1组(P0.05)。[结论]该新型大鼠脊髓打击器制作简便、可行性高,建立的SCI模型具有稳定性高、可重复性强、损伤程度可调节等特点,适合相关科研人员使用。
[Abstract]:Objective to prepare a simple and practical model device for spinal cord injury in rats. [methods] A new type of rat spinal cord punch was made with plank, new foam plate, suction tube, suture and metal pin. 36 adult female SD rats were selected. The body mass was 220 ~ 250g. The rats were randomly divided into sham-operated group (Sham group), light attack group (SCI1 group) and severe attack group (SCI2 group). The spinal cord injury model of rats was established by using self-made spinal cord batter. The SCI1 group was used 10 g 脳 25 mm strike specification. Direct strike injury group (SCI 2) used 10 g 脳 50 mm strike specification for vertical strike injury. The motor function of hind limbs was observed at 1 day before operation, 1 day before operation and 1 day after operation. The motor function of hind limbs was observed. The injured spinal cord tissues in each group were stained with HE and the neuronal nuclear antigen (Neu N) was detected by immunohistochemistry on the 28th day after operation. [results] the BBB score of Sham group was 21 at each observation time point, and the BBB score was 0 at 1 day after operation in each SCI group, and then the BBB score showed a trend of gradual recovery with the prolongation of time. The BBB score of SCI1 group was higher than that of SCI2 group from 3 days after operation (P 0.05). There was no significant difference in the left and right side movements of the hind limbs in each group, and no rats died during the experiment. On the 28th day after operation, the results of HE staining and Neu N immunohistochemical staining showed that the degree of spinal cord injury in SCI2 group was significantly worse than that in SCI1 group (P 0.05). [conclusion] the new rat spinal cord percussion device is simple and feasible. The SCI model has the characteristics of high stability, strong repeatability and adjustable injury degree, and is suitable for use by related researchers.
【作者单位】：南方医科大学附属广东省第二人民医院骨科;南方医科大学第三附属医院脊柱骨科;广州中医药大学第一附属医院中医内科;
【基金】：广东省科技计划项目(编号:2012B031800266) 广东省海珠区科技计划项目(编号:20130932)
【分类号】：R-332;R651.2

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