建立纤维环穿刺法椎间盘退变模型

发布时间：2018-05-18 03:09

本文选题：椎间盘退化 + 模型　；参考：《中国组织工程研究》2017年12期

【摘要】：背景:目前虽然纤维环穿刺法构建椎间盘退变模型的相关研究报道较多,但缺乏相对完整的影像、病理学以及细胞外基质改变(蛋白多糖含量)的系统对比性研究。所以经腹膜外入路暴露椎间隙纤维穿刺法造模的影像、病理学以及细胞外基质改变是否与人类椎间盘退变类似需进一步探讨。目的:验证采用纤维环穿刺法建立椎间盘退变模型的可行性。方法:将30只新西兰大白兔随机分为2组,每组15只。纤维环穿刺组在经腹膜外入路暴露L_(1,2),L_(2,3),_(3,4)椎间隙后,采用16号针头穿刺;而假手术组只做切开不做穿刺。于造模术后4,8,16周2组均随机抽取5只行MRI检查后,以空气栓塞法处死兔并切取椎间盘髓核组织,苏木精-伊红染色检查组织学变化,Safranin O染色观察髓核蛋白多糖水平改变。结果与结论:(1)MRI提示:纤维环穿刺组T2WI相椎间盘信号自造模术后开始降低,且随着术后时间延长T2WI相椎间盘信号降低越明显,于术后4周较假手术组有明显改变,术后16周最明显;假手术组术后各时间段改变不明显;(2)苏木精-伊红染色显示:纤维环穿刺组造模术后软骨样细胞减少,纤维样细胞增多且所占比例增大,细胞外纤维排列紊乱,于第4周较假手术组有明显改变,于第16周最明显;假手术组术后各时间段改变不明显;(3)Safranin O染色(髓核蛋白多糖水平):纤维环穿刺组组织着色于造模术后开始变浅,随着术后时间延长着色变浅越明显,于术后4周较假手术组有明显改变,术后16周最明显;(4)结果提示:纤维环穿刺法建立的椎间盘退变模型影像学表现及病理表现与人类椎间盘退变近似,提示纤维环穿刺法是建立椎间盘退变模型的可靠方法。
[Abstract]:Background: although there are many reports on the establishment of disc degeneration model by fiber loop puncture, there is a lack of systematic and comparative studies on the image, pathology and extracellular matrix changes (proteoglycan content). Therefore, the imaging, pathology and changes of extracellular matrix (ECM) of exposed intervertebral space fiber puncture via extraperitoneal approach need to be further explored. Objective: to verify the feasibility of establishing intervertebral disc degeneration model by fiber loop puncture. Methods: thirty New Zealand white rabbits were randomly divided into 2 groups with 15 rabbits in each group. In the fibrous loop puncture group, 16 needle puncture was used after the exposure of L1 / 2 / L / L / L / L / L / L / L / L / L / L / L / L through extraperitoneal approach, while in the sham operation group, only incision was made and no puncture was performed. After MRI examination, 5 rabbits in both groups were randomly selected at the 16th week after the operation. The rabbits were killed by air embolization and removed from the nucleus pulposus. The histopathological changes were examined by hematoxylin and eosin staining and the changes of nucleoproteoglycan levels were observed by Safranin O staining. Results and ConclusionMRI showed that the signal of intervertebral disc of T2WI phase in the fibrous annulus puncture group began to decrease after self-modeling, and the decrease of the signal of the intervertebral disc in T2WI phase was more obvious with the extension of postoperative time, and it was obviously changed at 4 weeks after operation compared with that in the sham operation group. The results of hematoxylin-eosin staining showed that chondroid cells decreased, fibroid cells increased and the proportion of fibroid cells increased in the sham operation group after 16 weeks. The arrangement of extracellular fibers was disordered, which was significantly changed in the 4th week than in the sham-operation group, and the most obvious in the 16th week. In the sham operation group, there was no obvious change of Safranin O staining at every time after operation (myelin proteoglycan level: the tissue of the fibrous ring puncture group began to become shallower after modeling, and the lighter the staining became with the extension of postoperative time. The results showed that the imaging and pathological manifestations of the degenerative model were similar to those of human intervertebral disc degeneration at 4 weeks after operation and 16 weeks after operation, and the pathological manifestations of the model were similar to those of human intervertebral disc degeneration. The results suggest that the method of fiber loop puncture is a reliable method to establish the model of disc degeneration.
【作者单位】：川北医学院附属医院骨科;
【基金】：四川省医学会创伤专项课题(2015GK007)~~
【分类号】：R-332;R681.5

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