血清二胺氧化酶含量与大鼠急性脊髓损伤后肠道运动功能的关系

发布时间：2018-05-18 03:05

本文选题：脊髓损伤 + 二胺氧化酶　；参考：《中国康复理论与实践》2017年06期

【摘要】：目的测定急性脊髓损伤大鼠血清中二胺氧化酶(DAO)的含量,同时观察肠道运动功能的变化和肠黏膜屏障损伤的程度。方法 45只Sprague-Dawley大鼠随机分为打击组(A组)、假手术组(B组)和对照组(C组),每组15只。采取改良Allen打击法(10 g×25 mm)损伤大鼠T_(10)脊髓节段。术后1 d、3 d、7 d分别对各组大鼠进行后肢功能BBB评分,测定大鼠回肠肌电慢波及平滑肌收缩,并做回肠HE染色,ELISA试剂盒测定血清DAO含量。结果脊髓损伤后1 d、3 d和7 d,A组BBB评分显著低于C组(P0.001),B组与C组无显著性差异(P0.05)。脊髓损伤后1 d、3 d,A组慢波频率和振幅低于C组(P0.05),收缩频率和振幅低于C组(P0.05),B组与C组无显著性差异(P0.05);脊髓损伤后7 d,三组肌电慢波频率和振幅、收缩的频率和振幅均无显著性差异(P0.05)。A组回肠黏膜水肿、倒伏,炎性细胞浸润,黏膜下间隙增大。A组肠黏膜损伤Chiu评分高于B组和C组(P0.05)。脊髓损伤后1 d、3 d,A组大鼠血清DAO含量高于C组(P0.05),B组与C组无显著性差异(P0.05);脊髓损伤后7 d,三组间血清DAO含量均无显著性差异(P0.05)。结论血清DAO含量可以反映大鼠急性脊髓损伤后肠道的运动功能和黏膜损伤程度。
[Abstract]:Objective to determine the content of diamine oxidase (DAO) in serum of rats with acute spinal cord injury (sci), and to observe the changes of intestinal motor function and the degree of intestinal mucosal barrier injury. Methods Forty-five Sprague-Dawley rats were randomly divided into two groups: group A (group A), group B (sham operation group) and group C (control group, 15 rats in each group). The modified Allen method was used to injure 10 g 脳 25 mm rat spinal cord segment. The functional BBB scores of hind limbs were measured on the 1st and 3rd day and 7 days after operation respectively. The ileal electromyography (EMG) and smooth muscle contraction (SMC) were measured, and the serum DAO levels were measured by HE staining Elisa kit. Results the BBB score of group A was significantly lower than that of group C on day 3 and day 7 after spinal cord injury. There was no significant difference between group B and group C (P 0.05). One day after spinal cord injury, the frequency and amplitude of slow wave in group A were lower than those in group C (P 0.05), and the contractile frequency and amplitude in group A were lower than those in group C (P 0.05), and there was no significant difference between group B and group C (P 0.05), and the frequency and amplitude of slow wave in group A were lower than those in group C at 7 days after spinal cord injury. There was no significant difference in the frequency and amplitude of contraction. The Chiu score of intestinal mucosal injury in group A was higher than that in group B and group C (P 0.05). The mucosal edema, lodging, inflammatory cell infiltration and submucosal space enlargement in group A were higher than those in group B and C. The serum DAO level in group A was higher than that in group C (P 0.05) at 1 d after spinal cord injury, and there was no significant difference between group B and group C (P 0.05) on the 7th day after spinal cord injury (P 0.05), but there was no significant difference in the content of serum DAO between the three groups on the 7th day after spinal cord injury (P 0.05). Conclusion Serum DAO level can reflect the intestinal motor function and the degree of mucosal injury after acute spinal cord injury in rats.
【作者单位】：成都医学院;
【分类号】：R651.2

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