瑞芬太尼控制性降压对大鼠肝缺血再灌注时脑组织iNOSmRNA表达的影响

发布时间：2018-05-21 18:23

本文选题：瑞芬太尼 + 控制性降压　；参考：《新疆医科大学》2017年硕士论文

【摘要】：目的:探讨瑞芬太尼控制性降压对大鼠肝缺血再灌注脑损伤的干预作用,是否对脑组织中的诱导型一氧化氮合酶(i NOS)mRNA表达造成影响。方法:健康雄性SD大鼠32只,体重280~320g,运用随机数字法,将其分为4组(n=8):假手术组(S组),仅游离肝蒂不阻断;肝缺血-再灌注组(I/R组),将肝左、中叶(70%)血供阻断60min后再灌注,建立大鼠部分肝缺血-再灌注模型;瑞芬太尼组(Rem组)与硝普钠组(SNP组)分别在开放肝门前5分钟静脉注入瑞芬太尼或硝普钠实施控制性降压,再灌注10min期间内维持MAP在70mmHg左右;再灌注24h处死全部大鼠,取脑组织,采用硝酸还原酶法测定一氧化氮(NO)含量,黄嘌呤法测定超氧化物歧化酶(SOD)活性,比色法测定丙二醛(MDA)含量,并以逆转录聚合酶链式反应(RT-PCR)方法检测脑组织iNOS mRNA的相对表达水平。结果:与S组相比,I/R组、Rem组、SNP组中MDA、NO含量及iNOS mRNA相对表达量升高,SOD含量降低,差异有统计学意义(P0.05);与I/R组相比,Rem组、SNP组MDA、NO含量及iNOS mRNA相对表达量降低,SOD含量升高(P0.05);与SNP组相比,Rem组MDA、NO含量及iNOS mRNA相对表达量降低,SOD含量升高(P0.05)。结论:瑞芬太尼进行控制性降压可减轻肝缺血再灌注导致的脑损伤,机制可能与降低NO水平,抑制iNOS mRNA表达等有关。
[Abstract]:Objective: To investigate the effect of controlled hypotension of remifentanil on cerebral ischemia-reperfusion injury in rats and the effect on the expression of inducible nitric oxide synthase (I NOS) mRNA in brain tissue. Methods: 32 healthy male SD rats, weighing 280~320g, were divided into 4 groups (n=8) by random number method: sham operation group (group S) and only free liver pedicle. No interruption; liver ischemia reperfusion group (group I/R), the left and middle lobe (70%) blood supply was blocked by 60min reperfusion, and rat partial liver ischemia-reperfusion model was established; remifentanil group (group Rem) and sodium nitroprusside group (group SNP) were injected intravenously with remifentanil or sodium nitroprusside for controlled hypotension in the group of sodium nitroprusside (group SNP), respectively, during the opening of the hepatic portal, and the internal dimension of the reperfusion during 10min. MAP was held around 70mmHg; reperfusion 24h was executed in all rats, the brain tissue was taken, the content of nitric oxide (NO) was measured by the nitrate reductase method, the activity of superoxide dismutase (SOD) was measured by xanthine method, the content of malondialdehyde (MDA) was measured by colorimetric method, and the relative expression water of iNOS mRNA in brain tissue was detected by reverse transcription polymerase chain reaction (RT-PCR) method. Results: compared with the S group, the content of MDA, NO and iNOS mRNA in group I/R, Rem and SNP increased, the content of SOD decreased, and the difference was statistically significant (P0.05). The amount of SOD increased (P0.05). Conclusion: the controlled hypotension of remifentanil can reduce the brain damage caused by hepatic ischemia reperfusion. The mechanism may be related to the reduction of NO level and the inhibition of the expression of iNOS mRNA.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R614

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