可同时释放两种骨形态发生蛋白复合支架的制备

发布时间：2018-05-21 19:37

本文选题：骨和骨组织 + 组织工程　；参考：《第二军医大学学报》2017年02期

【摘要】：目的研制一种可同时释放骨形态发生蛋白2(BMP-2)和骨形态发生蛋白7(BMP-7)的生物活性复合支架,以应用于骨组织工程的研究。方法分别采用复乳溶剂挥发法和相分离法制备负载BMP-2和BMP-7的聚乳酸/羟基乙酸-聚乙二醇(PGLA-PEG)微球和三维多孔的聚己内酯(PCL)支架。然后采用改良的二氯甲烷熏蒸法将PGLA-PEG微球黏附在PCL支架上,形成同时负载BMP-2和BMP-7的复合支架,并检测BMP-2和BMP-7的缓释效果。将人成骨细胞系hFOB1.19分别种植在复合支架和传统支架中,研究支架上的细胞增殖能力和成骨分化能力。结果制备的复合支架能同时缓慢地释放BMP-2和BMP-7。细胞培养第10天,复合支架上的细胞活性优于传统支架(P0.01),细胞形态正常。复合支架上细胞的碱性磷酸酶和Ⅰ型胶原蛋白、骨钙素、骨桥蛋白3种成骨基因的mRNA水平均高于传统支架(P0.05,P0.01)。结论成功研制出一种可同时释放BMP-2和BMP-7的生物活性复合支架,并可用于骨组织工程的研究。
[Abstract]:Objective to develop a bioactive composite scaffold for the simultaneous release of bone morphogenetic protein 2 (BMP-2) and bone morphogenetic protein 7 (BMP-7) in bone tissue engineering. Methods Polylactic acid / glycolic acid-polyethylene glycol (PGLA-PEG) microspheres loaded with BMP-2 and BMP-7 and three-dimensional porous poly (caprolactone) scaffolds were prepared by solvent volatilization and phase separation, respectively. Then the modified dichloromethane fumigation method was used to attach the PGLA-PEG microspheres to the PCL scaffold to form a composite scaffold loaded with both BMP-2 and BMP-7. The slow release effect of BMP-2 and BMP-7 was measured. Human osteoblast cell line hFOB1.19 was seeded into composite scaffold and traditional scaffold respectively to study the cell proliferation and osteogenic differentiation ability on the scaffold. Results the composite scaffolds could release BMP-2 and BMP-7 slowly at the same time. On the 10th day of cell culture, the cell activity of the composite scaffold was better than that of the traditional scaffold P0.01, and the cell morphology was normal. The mRNA levels of alkaline phosphatase, type I collagen, osteocalcin and osteopontin in the cells on the composite scaffold were higher than those in the traditional scaffold. Conclusion A bioactive composite scaffold which can release both BMP-2 and BMP-7 was successfully developed and can be used in bone tissue engineering.
【作者单位】：第二军医大学长海医院骨科;北方战区陆军65571部队卫生队;
【基金】：国家自然科学基金(81272026,8167090274)~~
【分类号】：R318.08;R68

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