骨肉瘤细胞和组织H3K27me3表达与临床病理特征相关性研究

发布时间：2018-05-25 02:17

本文选题：组蛋白修饰 + HKme　；参考：《中华肿瘤防治杂志》2016年22期

【摘要】：目的组蛋白H3K27三甲基化(H3K27me3)在肿瘤发生发展过程中起着重要作用,研究发现H3K27me3在肝癌和前列腺癌等恶性肿瘤中的表达和临床病理特征存在相关性,但其在骨肉瘤中的研究相对较少。本研究分析H3K27me3在骨肉瘤细胞和组织中的表达及其与临床病理的关系,并探讨H3K27me3在骨肉瘤发生和发展中的作用和意义。方法所有切片均来自于河北医科大学第四医院骨科2005-01-01-2011-01-01手术切除的标本。采用蛋白印迹法检测骨肉瘤MG-63、U2-OS、Sa-OS细胞系及hFOB1.19成骨细胞中H3K27me3表达的水平差异;采用免疫组织化学染色方法检测53例骨肉瘤组织及16例骨软骨瘤组织中H3K27me3的表达水平。Kaplan-Meier分析比较H3K27me3高表达和低表达患者生存率之间的差异。并应用Cox回归模型分析其表达水平对预后影响。结果hFOB1.19、MG-63、U2-OS和Sa-OS中H3K27me3蛋白表达水平分别为0.37±0.06、0.86±0.06、0.79±0.07和0.83±0.05,与hFOB1.19细胞中H3K27me3表达相比,3组骨肉瘤细胞系中H3K27me3蛋白表达水平均显著升高,P=0.023;与骨软骨瘤组织相比(56.3%),H3K27me3在骨肉瘤组织中高表达(84.9%),二者差异有统计学意义(P0.001),并与肿瘤大小、肺转移、Enneking分期和生存率有关,P值分别为0.037、0.020、0.023和0.046。且其表达水平与患者生存期显著相关,P=0.012。结论 H3K27me3在骨肉瘤细胞及骨肉瘤组织中呈高表达,并与肿瘤的临床病理特征相关,可能会是骨肉瘤患者重要的预后因子及治疗靶点。
[Abstract]:Objective the histone H3K27 trimethylated H3K27me3 (H3K27me3) plays an important role in tumorigenesis and development. The expression of H3K27me3 in malignant tumors such as liver cancer and prostate cancer is correlated with clinicopathological features. But its research in osteosarcoma is relatively few. In this study, we analyzed the expression of H3K27me3 in osteosarcoma cells and tissues and its relationship with clinicopathology, and discussed the role and significance of H3K27me3 in the development and development of osteosarcoma. Methods all the specimens were obtained from the orthopaedic department of the fourth Hospital of Hebei Medical University. The expression of H3K27me3 in osteosarcoma cell line MG-63U2-OS and hFOB1.19 osteoblast was detected by Western blot. Immunohistochemical staining was used to detect the expression of H3K27me3 in 53 cases of osteosarcoma and 16 cases of osteochondroma. Kaplan-Meier analysis was used to compare the survival rate of patients with high and low expression of H3K27me3. Cox regression model was used to analyze the effect of expression level on prognosis. Results the expression levels of H3K27me3 protein in HFOB1.19MG-63U2-OS and Sa-OS were 0.37 卤0.06, 0.86 卤0.06, 0.79 卤0.07 and 0.83 卤0.05, respectively. The expression of H3K27me3 protein in osteosarcoma cell lines was significantly higher than that in hFOB1.19 cells, and H3K27me3 in osteosarcoma was significantly higher than that in osteochondroma. The difference between them was statistically significant (P 0.001) and the size of the tumor was higher than that of the tumor. The P values of Enneking stage and survival rate were 0.037 0. 020 0. 023 and 0. 046 6 respectively. There was a significant correlation between the expression level and the survival time of the patients. Conclusion H3K27me3 is highly expressed in osteosarcoma cells and osteosarcoma tissues and may be an important prognostic factor and therapeutic target in osteosarcoma patients.
【作者单位】：河北医科大学第四医院骨科;河北医科大学第四医院心内科;河北医科大学第四医院病理科;河北医科大学第四医院科研中心;
【基金】：河北省卫生厅医学科学研究重点课题计划(20160159) 河北省自然基金面上项目(H2015206314)
【分类号】：R738.1

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