艾芬地尔预处理对七氟醚导致的幼年大鼠学习记忆和认知能力损伤的保护作用及机制

发布时间：2018-05-25 02:27

本文选题：七氟醚 + N-甲基-D-天冬氨酸亚型B受体　；参考：《临床麻醉学杂志》2017年07期

【摘要】：目的观察N-甲基-D-天冬氨酸亚型受体(NR2B)选择性拮抗剂艾芬地尔对七氟醚导致的幼年大鼠学习、记忆和认知能力损伤的保护作用及机制。方法 SD大鼠28只,7日龄,体重15~18g,采用随机数字表法,将大鼠分为四组,每组7只:对照组(C组),腹腔注射生理盐水0.2 ml;艾芬地尔组(I组),腹腔注射N-甲基-D-天冬氨酸(NMDA)亚型受体NR2B选择性拮抗剂艾芬地尔5.0mg/kg;七氟醚组(S组),吸入七氟醚前2h腹腔注射生理盐水0.2ml,持续吸入2.0%七氟醚4h;艾芬地尔+七氟醚组(IS组),对应时点腹腔注射艾芬地尔5.0 mg/kg,持续吸入2.0%七氟醚4h。给药后3周处死大鼠,取其海马切片,行电生理实验,在Schaffer侧支上给予高频成串刺激(100Hz,4个脉冲),待值群峰电位(population spike,PS)稳定后,每隔10分钟检测单通道刺激后值群峰电位振幅(population spike amplitude,PSA)值和记录长时程增强(long-term potentiation,LTP),诱发成功情况。结果与C组比较,在单通道刺激后各时点S组PSA值明显减小,LTP诱发成功率明显降低(P0.01);与S组比较,在单通道刺激后各时点IS组PSA值明显增大,LTP诱发成功率明显升高(P0.01)。结论 NR2B受体参与七氟醚导致的新生大鼠认知功能障碍,艾芬地尔5.0mg/kg预处理有助于改善其神经毒性,产生脑保护作用。
[Abstract]:Aim to investigate the protective effect and mechanism of epinfandil, a selective antagonist of N-methyl-Daspartic acid subtype receptor (NR2B), on learning, memory and cognitive impairment induced by sevoflurane in young rats. Methods 28 Sprague-Dawley rats were divided into four groups by random digital table method. Seven rats in each group were given intraperitoneal injection of normal saline (0.2 ml) in control group C (n = 7); group I (n = 1) received intraperitoneal injection of epinfandil (5.0 mg / kg), a selective antagonist of NR2B subtype of N-methyl-Daspartate (NMDAA); and sevoflurane group (group S, inhaled sevoflurane). Two hours before, 0.2 ml of normal saline was injected intraperitoneally and 2.0% sevoflurane was inhaled continuously for 4 h, and the group of ifedil sevoflurane was injected intraperitoneally at the corresponding time point of 5.0 mg / kg, and 2.0% sevoflurane for 4 h. Three weeks after administration, the rats were killed, the hippocampal slices were taken and electrophysiological experiments were performed. The rats were treated with high frequency strands stimulation of 100Hz on the lateral branches of Schaffer, 4 pulses, and the population spike-PSs were stabilized. The population spike amplitudes were measured every 10 minutes after single channel stimulation, and long term potentiation was recorded to induce success. Results compared with group C, the success rate of PSA in group S was significantly lower than that in group C at each time point after single channel stimulation, and that in group S was significantly higher than that in group S at each time point after single channel stimulation, and the success rate of PSA in group S was significantly higher than that in group S at each time point after single channel stimulation (P 0.01). Conclusion NR2B receptor is involved in cognitive impairment induced by sevoflurane in neonatal rats. 5.0mg/kg preconditioning with ifedil can improve neurotoxicity and produce brain protection.
【作者单位】：青岛大学医学院麻醉学系;济宁市第一人民医院麻醉科;济宁市任城区第一妇幼保健院产科;济南千佛山医院麻醉科;
【分类号】：R614

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