光动力联合抗生素治疗大鼠创伤感染

发布时间：2018-06-07 04:50

本文选题：医用光学 + 光动力抗菌疗法　；参考：《激光与光电子学进展》2017年03期

【摘要】：探讨了新型卟啉类光敏剂PA1介导的光动力抗菌(PACT)联合抗生素治疗重度创伤感染大鼠的效果并简要探讨其机制。健康雄性Wistar大白鼠24只(清洁级)作为实验对象,建立大面积创伤感染模型,随机分成模型对照组、PACT治疗组、抗生素治疗组、PACT联合抗生素治疗组,每组6只,共治疗8d。检测不同时间点各组创面下的菌落数和创面愈合率,并采用酶联免疫吸附测定(ELISA)方法检测创面周边组织碱性成纤维因子(bFGF)、肿瘤坏死因子α(TNF-α)和白介素6(IL-6)的表达。结果表明PACT联合抗生素治疗与模型对照组相比能显著减轻大鼠创伤组织的炎症,减少创面组织中细菌的数量,加快创面愈合速率,缩短创面愈合时间;同时还能提高创面组织中bFGF的含量,抑制组织中炎症相关因子TNF-α和IL-6的表达,其效果优于单用PACT的治疗组和单用抗生素的治疗组。结果表明PACT联合抗生素可以治疗大面积创伤感染。
[Abstract]:The therapeutic effect of a new porphyrin Guang Min agent PA1 mediated photodynamic antibacterial agent (PA1) combined with antibiotics in the treatment of severe traumatic infection in rats was studied and its mechanism was briefly discussed. Twenty-four healthy male Wistar rats (clean grade) were randomly divided into two groups: the model control group treated with pat, the antibiotic group treated with pat combined with antibiotics, each group (6 rats) for 8 days. The colony number and wound healing rate were measured at different time points. The expression of basic fibroblast factor (bFGF- 伪), tumor necrosis factor- 伪 (TNF- 伪) and interleukin-6 (IL-6) were detected by Elisa. The results showed that PACT combined with antibiotics could significantly reduce the inflammation of wound tissue, reduce the number of bacteria in the wound tissue, accelerate the wound healing rate and shorten the wound healing time compared with the model control group. At the same time, it could increase the content of bFGF and inhibit the expression of TNF- 伪 and IL-6 in the wound tissue. The effect was better than that of the group treated with PACT alone and the group treated with antibiotics alone. The results showed that PACT combined with antibiotics could be used to treat extensive trauma infection.
【作者单位】：河北大学医学院;河北大学预防医学与卫生事业管理系;河北大学附属医院;中国医学科学院生物医学工程研究所;
【基金】：天津市重大科技支撑项目(12ZCDZSY11900) 河北大学医学学科建设项目(2015A2001);河北大学大学生创新训练计划(2016149)
【分类号】：R641

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